| |
|
|
|
Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
|
|
|
|
Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
|
|
Taq DNA Polymerase with 10X Taq Reaction Buffer
- Recombinant source
- Functionality preserved at wide range of temperatures
- Highly reliable and reproducible reactions
- Supplied with detergent-free Reaction Buffer for compatibility with microarray and DHPLC applications
- Compatible with the use of modified nucleotides (dUTP, dITP, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
- Available in 2 different concentrations: 5 units/µL and 1 unit/µL
|
 |
|
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The enzyme is supplied with detergent-free 10X Taq Reaction Buffer, making it compatible with microarray and DHPLC applications.
Norgen’s Taq DNA Polymerase with 10X Taq Reaction Buffer is available in 2 convenient sizes:
- 1000 units at a concentration of 5000 units/mL (Cat #28088)
- 200 units at a concentration of 1000 units/mL (Cat #28094)
Enzyme Source:
An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1 Applications
- Routine PCR
- Preparation of PCR products for cloning
- Primer extension
- Microarray analysis
- DHPLC
- DNA labeling
- DNA sequencing
|
|
|
|
|
| DATA
|
 |
Figure 1. Successful Amplification of Different Sized Amplicons. Norgen’s Taq DNA Polymerase was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. Norgen’s Taq DNA Polymerase was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is Norgen’s PCR Sizer 100 bp DNA Ladder and Lane M2 is Norgen’s MidRanger 1kb DNA Ladder. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Reagents supplied - Cat# 28088:
- 1000 units of Taq DNA Polymerase (5000 units/mL)
- 4 x 1.25 mL Taq Reaction Buffer (10X)
Reagents supplied - Cat# 28094:
- 200 units of Taq DNA Polymerase (1000 units/mL)
- 1 x 1.25 mL Taq Reaction Buffer (10X)
Reaction Conditions:
1X Taq Reaction Buffer (10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, PCR stabilizer, pH 8.3 @ 25°C)
Storage Buffer:
50 mM Tris-HCl pH 8 at 25°C, 50 mM KCl, 50mM NaCl, 0.5 mM Dithiothreitol, 0.1 mM EDTA, 50% Glycerol, 1% Triton X-100
Storage Temperature:
-20°C
|
|
| PROTOCOL |
Taq DNA Polymerase with 10X Taq Reaction Buffer - 5000 units/mL - Protocol
PDF version
|
Taq DNA Polymerase with 10X Taq Reaction Buffer - 1000 units/mL - Protocol
PDF version
|
| |
| PRODUCT INFORMATION SHEETS |
Taq DNA Polymerase with 10X Taq Reaction Buffer - Product Information Sheet
|
| |
| APPLICATION NOTES |
Taq DNA Polymerase with 10X Taq Reaction Buffer - Frequently Asked Questions
|
| |
POSTERS |
| |
| MSDS |
| |
| SUPPLEMENTARY PROTOCOLS |
| |
| REFERENCES |
Taq DNA Polymerase with 10X Taq Reaction Buffer - References
HTML
|
| |
| FREQUENTLY ASKED QUESTIONS |
|
Introductory 50% Discount on PCR Master Mixes for a LIMITED TIME
Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
Taq DNA Polymerase with 10X Taq Reaction Buffer |
28088 |
|
Buy Now |
Taq DNA Polymerase with 10X Taq Reaction Buffer |
28094 |
200 units (1 U/uL) |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
|
|
|
|
|
|
