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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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Blood Genomic DNA Isolation Maxi Kit
(Cat# 31200)
For the rapid preparation of high quality DNA from whole blood
- Input volumes of 3 mL to 10 mL
- No phenol required for DNA isolation
- Fast and convenient spin column protocol
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Norgen’s Blood Genomic DNA Isolation Maxi Kit is designed for the rapid preparation of genomic DNA from 2 mL up to 10 mL of whole blood.
Principle
Purification is based on spin column chromatography. Briefly, the sample is lysed and the released genomic DNA is bound to Norgen's column under optimized salt concentrations. Under these conditions only the genomic DNA is bound to Norgen's column while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities. Lastly, the purified genomic DNA is eluted into the provided Elution Buffer.
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Performance
The Blood Genomic DNA Isolation Maxi Kit allows for the isolation of genomic DNA from the blood of various species, including humans. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. Preparation time for a single sample is 50-70 minutes, and each kit contains sufficient materials for 12 preparations. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including PCR and Southern Blot analysis.
FEATURES AND BENEFITS
- No Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals
- Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 50-70 minutes.
- Recovered genomic DNA is suitable for downstream applications -Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis.
- High quality DNA with no RNA contamination - No contamination or degradation of the isolated genomic DNA is observed.
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| APPLICATIONS
Genomic DNA isolated using the Blood Genomic DNA Isolation Maxi Kit is well-suited to a wide range of downstream applications including:
- qPCR
- PCR
- Sequencing
- Restriction Digests
- Southern blotting
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Figure 1. High Yields of Genomic DNA Isolated from Whole Blood. Genomic DNA was isolated from two different 2 mL whole blood samples using Norgen's Blood Genomic DNA Isolation Maxi Kit. Following isolation, 10 µL from each 1 mL elution was loaded on 1% TAE agarose gel. Lanes 1 and 3 correspond to the first elution, while Lanes 2 and 4 correspond to the second elution. Norgen's Blood Genomic DNA Isolation Maxi Kit demonstrated a high yield of intact genomic DNA.
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Figure 2. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. Genomic DNA was isolated from 10 mL of whole human blood using Norgen's Blood Genomic DNA Isolation Maxi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 1mL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control. |
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Kit Specifications
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Maximum Blood Input
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10 mL
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Column Binding Capacity
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> 500 ug
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Average Yield (200 uL of blood)
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200-600 µg*
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Elution Volume
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0.6-1 mL
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| Analyte Purified |
Genomic DNA, mitochondrial DNA, viral DNA |
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Format
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Spin Columns
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| Time to Complete 10 Purifications |
50-70 minutes |
*Yield will vary depending on the type of blood processed
Kit Contents
1. Lysis Solution
2. Wash Solution I
3. Wash Solution II
4. Elution Buffer
5. Proteinase K
6. Maxi Spin Columns
7. Collection tubes
8. Elution tubes
9. Product Insert
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended. |
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| PROTOCOL |
Blood Genomic DNA Isolation Maxi Kit - Protocol
PDF version
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| PRODUCT INFORMATION SHEETS |
Blood Genomic DNA Isolation Maxi Kit - Product Information Sheet
PDF version
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| APPLICATION NOTES |
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POSTERS |
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| MSDS |
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| SUPPLEMENTARY PROTOCOLS |
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| REFERENCES |
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| FREQUENTLY ASKED QUESTIONS |
Frequently Asked Questions
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
Blood Genomic DNA Isolation Maxi Kit |
31200 |
12 Preps |
Buy Now |
Blood Genomic DNA Isolation Maxi Kit |
31200 |
12 Preps |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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