| |
|
|
|
Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
|
|
|
|
Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
|
|
|
For precise sizing of RNA molecules using native or denaturing gels
- Eight discrete bands, ranging from 100 to 1,000 bases
- Higher intensity band at 500 bases for easy reference
- No need for staining or destaining as loading dye contains ethidium bromide
- Convenient lyophilized format provides better product stability
|
|
The Norgen 100b RNA ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.
Contents
- 2 vials of lyophilized RNA ladder (25 loads each).
- 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution.
- 2x loading buffer.
|
|
|
FEATURES AND BENEFITS
- Eight discrete bands, ranging from 100 to 1,000 bases
- Higher intensity band at 500 bases for easy reference
|
|
|
|
|
| APPLICATIONS
This ladder is ideal for precise sizing of a wide range of RNA molecules using native or denaturing gels. |
 |
Figure 1: 100 b RNA Ladder |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Specifications:
- Eight discrete bands, ranging from 100 to 1,000 bases
- Higher intensity band at 500 bases for easy reference
Contents:
- 2 vials of lyophilized RNA ladder (25 loads each).
- 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution.
- 2x loading buffer.
Instructions:
To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.
Storage:
Store at -20°C.
|
|
Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
100b RNA Ladder |
15002 |
50 loads |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
|
|
|
|
|
|
