Norgen’s Urine DNA Isolation Micro Kit provides a fast, reliable and simple procedure for isolating DNA from as low as 50 µL of urine up to 2 mL of urine. Many advantages favour the use of urinary DNA for cancer biomarker discovery and diagnosis of different pathogens over the use of blood and tissue samples. DNA found in urine can be divided into 2 basic categories. The larger species genomic-DNA (gDNA) is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid. Both types of DNA can be isolated reliably using this kit. Typical yields of DNA isolated will vary depending on the input sample, with more concentrated samples tending to yield more DNA.
Purification is based on spin column chromatography using Norgen's proprietary resin as the separation matrix. Briefly, urine DNA is bound to the column in the presence of Binding Solution (BIND). This is folllowed by washing of the bound DNA to remove the remaining proteins or other impurities (WASH). Lastly, the purified total urine DNA is eluted into the provided Elution Buffer (ELUTE). Please see the procedure flowchart to the right.
The kit allows for the removal of highly concentrated salts, metabolic wastes and proteins, to allow for the isolation of high quality, concentrated DNA. Preparation time for a single sample is about 30 minutes. The purified urine DNA is compatible with most of the molecular biological applications such as PCR, q-PCR and DNA fingerprinting
FEATURES AND BENEFITS
- Fast processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes.
- Isolation of both types of urine DNA - Isolate both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation).
- High quality DNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated DNA to be used in various downstream applications.
- Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with PCR, q-PCR and DNA fingerprinting