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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.

As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.

It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs.

Please contact us at:
info@norgenbiotek.com

Norgen Biotek Corp.
3430 Schmon Parkway, Thorold
Ontario, Canada, L2V 4Y6

Tel: (905) 227-8848
Fax: (905) 227-1061

HighRanger 1 kb DNA Ladder

Publications

(Cat# 11900)

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price of $39.00 each.
Contact us for details.

Ideal for size determination of digested DNA

Ready-to-use
Quantitative
Precise
Highly Stable
Thirteen discrete fragments ranging from 300bp to 10000bp
Higher intensity reference band at 5000bp

Product Description
Product Description
Applications & Data
Applications & Data
Specifications
Specifications
Resources
Resources
Ordering
Ordering

The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

Ready-to-use
Quantitative
Precise
Highly Stable

APPLICATIONS

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band. Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: HighRanger 1 kb DNA Ladder

HighRanger 1kb DNA Ladder (Cat# 11900) - 100 loads

Ladder Properties:
• Thirteen discrete bands, ranging from 300bp to 10,000bp.
• Higher intensity band at 5000bp for easy reference.

Fragment	Size (bp)	Mass (ng)
1	10000	58
2	8000	53
3	6000	46
4	5000	67
5	4000	56
6	3000	47
7	2500	41
8	2000	34
9	1500	29
10	1000	18
11	700	13
12	500	23
13	300	14

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage:

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

PROTOCOL

HighRanger 1 kb DNA Ladder - Product Insert
PDF version

PRODUCT INFORMATION SHEETS

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders
HTML | PDF version

POSTERS

MSDS

HighRanger 1 kb DNA Ladder - MSDS
PDF version

SUPPLEMENTARY PROTOCOLS

REFERENCES

HighRanger 1 kb DNA Ladder - References

FREQUENTLY ASKED QUESTIONS

3430 Schmon Parkway, Thorold, ON L2V 4Y6 Canada
Phone: (905) 227-8848 | Toll Free: 1-866-NORGENB (667-4362) | Fax: (905) 227-1061 | email: info@norgenbiotek.com