PCR Ranger 100 bp DNA Ladder (Cat# 11300) - 100 loads
Ladder Properties:
• Eleven discrete bands, ranging from 50bp to 1,000bp.
• Higher intensity band at 500bp for easy reference.
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
1000
|
91
|
|
2
|
900
|
66
|
|
3
|
800
|
61
|
|
4
|
700
|
51
|
|
5
|
600
|
43
|
|
6
|
500
|
70
|
|
7
|
400
|
28
|
|
8
|
300
|
23
|
|
9
|
200
|
30
|
|
10
|
100
|
22
|
|
11
|
50
|
15
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer. |
