Fungi/Yeast Genomic DNA Isolation Kit

Figure 1. DNA Isolation from Different Fungi Species and Yeast.   To demonstrate the purification of DNA from different fungal species, 30 mg of fungi were collected from plate cultures of Pichia sp, Aspergillus niger, Cladosporium cladasporioides, Botrytis cinerea and Mucor racemosus, and the DNA was extracted using Norgen’s Fungi/Yeast Genomic DNA Isolation kit. The bead system efficiently lysed the fungal cell walls with the provided Lysis Solution, and total DNA was eluted in 100 µL. For analysis, 10 µL from each elution was loaded in 1% 1xTAE agarose gel. Lane 1: Yeast (Pichia sp.), Lane 2: Aspergillus Niger; Lane 3: Cladosporium cladosporioides; Lane 4: Botrytis cinerea; Lane 5: Mucor racemosus; Lane M: Norgen’s HighRanger 1kb DNA Ladder. The optional RNase treatment was not performed during the process.

Figure 2.  DNA can be Isolated from Spores and Mycelium.   Genomic DNA was extracted from the spores and mycelium of Botrytis cinera using Norgen's Fungi/Yeast Genomic DNA Isolation Kit.  For the spores, 10⁵ spores/mL were collected from a plate culture by adding 3 mL of 0.9% NaCl to the fungi culture plate and gently shaking to extract the spores. For mycelium, 20 mg of mycellium was collected with sterilized forceps. DNA was isolated using Norgen’s Fungi/Yeast Genomic DNA Isolation Kit with the provided Bead Tubes. For analysis, 10 µL from each 100 µL elution was loaded on a 1% 1xTAE agarose gel. Lane M: Norgen’s HighRanger 1kb DNA Ladder. Optional RNase treatment was not performed during the extraction.  As it can be seen, DNA could be successfully isolated from both the spores and mycelium.



Figure 3. Identification of Fungal and Yeast Species in a Real-time PCR (SYBR Green).   DNA was isolated from 50 mg samples (wet weight) of yeast (Pichia sp) and fungi including Aspergillus niger, Cladosporium cladasporioides, Botrytis cinerea and Mucor racemosus using Norgen’s Fungi/Yeast Genomic DNA Isolation Kit, and 2 µL of each of the 100 µL DNA elutions was used in a qPCR (SYBR Green) reaction with specific fungal and yeast primers. The qPCR was successful in amplifying and detecting all the yeast and fungal DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.