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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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CloneSizer 100 bp DNA Ladder
(Cat# 11600)
DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price of $39.00 each.
Contact us for details.
Includes a 2686bp (pUC19) reference band for easy clone identification
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Fourteen discrete fragments ranging from 100bp to 2000bp in 100bp increments
- Higher intensity reference bands at 500bp and 1000bp
- 2686bp (pUC19) reference band for easy clone identification
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The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100bp to 2000bp in 100bp increments, double intensity reference bands at 500 and 1000bp and an additional 2686bp (pUC19) reference band for easy clone identification.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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FEATURES AND BENEFITS
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
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| APPLICATIONS
Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band. Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products. |
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Figure 1: CloneSizer 100 bp DNA Ladder |
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CloneSizer 100 bp DNA Ladder (Cat# 11600) - 100 loads
Ladder Properties:
- Fourteen discrete fragments ranging from 100bp to 2000bp in 100bp increments
- Higher intensity reference bands at 500bp and 1000bp
- 2686bp (pUC19) reference band for easy clone identification
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Fragment
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Size (bp)
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Mass (ng)
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1
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2686
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2
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2000
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53
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3
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1500
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41
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4
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1200
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42
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5
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1000
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56
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6
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900
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30
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7
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800
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29
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8
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700
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25
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9
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600
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25
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10
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500
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52
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11
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400
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19
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| 12 |
300 |
20
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| 13 |
200 |
19
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| 14 |
100 |
17
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Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
Clone Sizer 100bp DNA Ladder |
11600 |
100 loads |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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