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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.

As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.

It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs.

Please contact us at:
info@norgenbiotek.com

Norgen Biotek Corp.
3430 Schmon Parkway, Thorold
Ontario, Canada, L2V 4Y6

Tel: (905) 227-8848
Fax: (905) 227-1061

FullRanger 100 bp DNA Ladder

Publications

(Cat# 11800)

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price of $39.00 each.
Contact us for details.

Ready-to-use
Quantitative
Highly Stable
Precise
Sixteen discrete fragments ranging from 100bp to 5000bp
Higher intensity reference bands at 500bp and 1000bp

Product Description
Product Description
Applications & Data
Applications & Data
Specifications
Specifications
Resources
Resources
Ordering
Ordering

The Norgen FullRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our FullRanger contains sixteen discrete fragments ranging from 100bp to 5000bp with higher intensity reference bands at 500bp and 1000bp. This Ladder is ideal for accurate visual determination in both large and small cloning applications.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

Ready-to-use
Quantitative
Highly Stable
Precise

APPLICATIONS

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band. Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: FullRanger 100 bp DNA Ladder

FullRanger 100 bp DNA Ladder (Cat# 11800) - 100 loads

Ladder Properties:
• Sixteen discrete bands, ranging from 100bp to 5,000bp.
• Higher intensity reference bands at 500bp and 1000bp

Fragment	Size (bp)	Mass (ng)
1	5000	55
2	4000	44
3	3000	33
4	2500	28
5	2000	22
6	1500	17
7	1000	50
8	900	35
9	800	31
10	700	43
11	600	24
12	500	56
13	400	16
14	300	22
15	200	15
16	100	11

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage:

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

PROTOCOL

FullRanger 100 bp DNA Ladder - Product Insert
PDF version

PRODUCT INFORMATION SHEETS

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders
HTML | PDF version

POSTERS

MSDS

FullRanger 100 bp DNA Ladder - MSDS
PDF version

SUPPLEMENTARY PROTOCOLS

REFERENCES

FullRanger 100 bp DNA Ladder - References

FREQUENTLY ASKED QUESTIONS

3430 Schmon Parkway, Thorold, ON L2V 4Y6 Canada
Phone: (905) 227-8848 | Toll Free: 1-866-NORGENB (667-4362) | Fax: (905) 227-1061 | email: info@norgenbiotek.com