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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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HighRanger Plus 100 bp DNA Ladder
(Cat# 12000)
DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price of $39.00 each.
Contact us for details.
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Nineteen discrete fragments ranging from 100bp to 10000bp
- Higher intensity reference bands at 500bp, 1000bp and 5000bp
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The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100bp to 10000bp with higher intensity reference bands at 500bp, 1000bp and 5000bp. This Ladder is ideal for size determination of digested DNA.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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FEATURES AND BENEFITS
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
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| APPLICATIONS
Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band. Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products. |
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Figure 1: HighRanger Plus 100 bp DNA Ladder |
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HighRanger Plus 100 bp DNA Ladder (Cat# 12000) - 100 loads
Ladder Properties:
- Nineteen discrete fragments ranging from 100bp to 10000bp.
- Higher intensity bands at 500bp, 1000bp and 5000bp for easy reference.
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Fragment
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Size (bp)
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Mass (ng)
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1
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10000
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42
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2
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8000
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33
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3
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6000
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25
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4
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5000
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42
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5
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4000
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35
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6
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3000
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27
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7
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2500
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22
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8
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2000
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18
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9
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1500
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13
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10
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1000
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40
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11
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900
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28
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| 12 |
800 |
25
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| 13 |
700 |
34
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| 14 |
600 |
19
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| 15 |
500 |
44
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| 16 |
400 |
13
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| 17 |
300 |
18
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| 18 |
200 |
13
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| 19 |
100 |
10
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Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer. |
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| PROTOCOL |
HighRanger Plus 100 bp DNA Ladder - Product Insert
PDF version
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| PRODUCT INFORMATION SHEETS |
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| APPLICATION NOTES |
DNA Quantification by Gel Densitometry with Norgen DNA Ladders
HTML
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PDF version
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POSTERS |
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| MSDS |
HighRanger Plus 100 bp DNA Ladder - MSDS
PDF version
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| SUPPLEMENTARY PROTOCOLS |
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| REFERENCES |
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| FREQUENTLY ASKED QUESTIONS |
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
HighRanger Plus 100bp DNA Ladder |
12000 |
100 loads |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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