MidRanger 1kb DNA Ladder (Cat# 11700) - 100 loads
Ladder Properties:
• Ten discrete fragments ranging from 300bp to 5000bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
5000
|
88
|
|
2
|
4000
|
78
|
|
3
|
3000
|
67
|
|
4
|
2500
|
58
|
|
5
|
2000
|
50
|
|
6
|
1500
|
43
|
|
7
|
1000
|
|
|
8
|
700
|
34
|
|
9
|
500
|
33
|
|
10
|
300
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
|
