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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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ProteoLadder 125 (14.7 kDa - 125 kDa)
(Cat# 12510)
For precise sizing of proteins using SDS-PAGE
- Ready-to-use and highly stable
- Recombinant proteins of known amino acid sequence and mass
- 8 discrete protein bands from 14.7 kDa to 125 kDa
- Sufficient amount to load 100 gel lanes
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The ProteoLadder 125 Ladder is ideal for the accurate molecular weight sizing of proteins by SDS-PAGE. The proteins separate into concise bands from 14.7 kDa to 125 kDa when analyzed by SDS-PAGE and stained with Coomassie Blue.
Contents
0.5 mL of ready-to-use mix comprised of 8 purified recombinant proteins in loading buffer (50mM Tris HCl, pH 7.5, 100mM DTT, 0.01% Bromophenol Blue, 1mM EDTA, 1mM Sodium Azide, 2% SDS and 20% Glycerol). FEATURES AND BENEFITS
- Ready-to-use
- Highly stable
- 8 discrete protein bands from 14.7 kDa to 125 kDa
- Precise
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| APPLICATIONS
This ladder is ideal for precise sizing of a wide range of proteins when analyzed by SDS-PAGE and stained with Coomassie Blue. |
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Figure 1: ProteoLadder 125 (14.7 kDa - 125 kDa)
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Specifications:
- 8 discrete protein bands from 14.7 kDa to 125 kDa
Contents:
- 0.5 mL of ready-to-use mix comprised of 8 purified recombinant proteins in loading buffer (50mM Tris HCl, pH 7.5, 100mM DTT, 0.01% Bromophenol Blue, 1mM EDTA, 1mM Sodium Azide, 2% SDS and 20% Glycerol).
Instructions:
- Thaw ladder at room temperature. Check contents to ensure absence of precipitated solids prior to gel loading. Heat at 95oC for 5 minutes. For best results, load 5 µL of marker per mini-gel well (1 mm gel thickness).
Storage:
- -20oC is recommended for storage. It is advisable to dispense the ladder into smaller volumes to reduce the potential of contaminating the stock and the effects of repeated freezing and thawing
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| PROTOCOL |
ProteoLadder 125 (14.7 kDa - 125 kDa) - Protocol
PDF version
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| PRODUCT INFORMATION SHEETS |
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| APPLICATION NOTES |
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POSTERS |
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| MSDS |
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| SUPPLEMENTARY PROTOCOLS |
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| REFERENCES |
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| FREQUENTLY ASKED QUESTIONS |
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
ProteoLadder 125 |
12510 |
100 loads |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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