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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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Saliva DNA Isolation Kit
(Cat# 45400)
For the rapid purification of high-quality DNA from preserved saliva samples
- Sample collection is non-invasive and painless
- Fast and easy processing using a rapid spin-column format
- Isolate high quality genomic DNA
- Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices
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Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. The isolated DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis. This kit can be used to isolate saliva DNA for in vitro diagnostic use.
The process first involves the collection and preservation of the saliva samples using Norgen’s Saliva DNA Collection and Preservation Devices, part of Norgen’s Saliva DNA Collection, Preservation and Isolation Kit (#35700). The resulting saliva sample can be processed immediately, or alternatively can be stored at room temperature for over 2 years prior to DNA isolation. When DNA isolation is required, Proteinase K and Binding Solution are then added to the sample, followed by the addition of ethanol. The resulting solution is loaded onto a spin-column (BIND). Under these conditions only the genomic DNA will bind to the column while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified genomic DNA is eluted into 200 µL of the provided Elution Buffer (ELUTE). Please see the procedure flowchart to the right.
Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from preserved saliva samples. FEATURES AND BENEFITS
- Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 30 minutes.
- Sample collection is non-invasive and painless - Saliva offers an attractive alternative to blood or tissue for the isolation of human DNA samples for use in diagnostic applications.
- Compatible with Norgen's collection and preservation products - This product allows for the isolation of DNA from samples collected and preserved using Norgen's Saliva DNA Collection and Preservation Devices
- Recovered DNA is suitable for various downstream applications - Purified saliva DNA can be used in a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis. This kit can also be used to isolate saliva DNA for in vitro diagnostic use.
Download our brochure.
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APPLICATIONS
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PCR
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Southern Blot Analysis
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Sequencing
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Microarray Analysis
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In vitro diagnostic use.
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Figure 1. High Quality and Yield of DNA from Saliva Samples. Total DNA was isolated from 250 µL of eight different saliva samples using Norgen’s Saliva DNA Isolation Kit (Lanes 1-8). For evaluation, 20 µL of each 100 µL DNA elution was run on a 1.0% agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M: Norgen’s HighRanger 1kb DNA Ladder. |
Figure 2. Detection of 5S rDNA from Saliva Samples. Norgen's Saliva DNA Isolation Kit was used to isolate DNA from 250 µL samples of saliva mixed with equal amounts of Lysis Solution. From the 100 µL elution, 1 µL, 3 µL, 6 µL and 9 µL of saliva DNA was directly mixed to real-time PCR master mix (total reaction volume 20 µL), and the real-time PCR reaction was performed. 5S rDNA was successfully amplified from the different template volumes without any inhibition, indicating the excellent quality of saliva DNA for down stream applications. |
Figure 3. Real-time PCR Consistency from Saliva Samples. Norgen's Saliva DNA Isolation Kit was used to isolate DNA from 250 µL samples of saliva mixed with equal amounts of Lysis Solution. Eight samples were used from same donor. From the 100 µL elution, 5 µL of saliva DNA was directly mixed to real-time PCR master mix (total reaction volume 20 µL), and the real-time PCR reaction was performed. GAPDH gene was successfully amplified from the different samples without any inhibition and at the same Ct, indicating the isolation consistency and the excellent quality of saliva DNA for down stream applications.
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Kit Specifications
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Number of preps
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50
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Maximum Saliva Input
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0.5 mL preserved saliva
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Average Yield from 0.25 mL of Saliva*
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7 ug
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Average purity (OD260/280)
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1.8 - 2.1
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Time to Complete 10 Purifications
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90 minutes (30 minutes hands on)
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* Average yield will depending on the donor
Saliva DNA Isolation Kit Contents
1. Proteinase K
2. Binding Solution
3. Wash Solution
4. Elution Buffer
5. Spin Columns
6. Elution Tubes
7. Product Insert
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The Proteinase K can be stored at either room temperature or 4oC.
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| PROTOCOL |
Saliva DNA Isolation Kit - Protocol
PDF version
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| PRODUCT INFORMATION SHEETS |
Saliva DNA Isolation Kit - Product Information Sheet
PDF version
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| APPLICATION NOTES |
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POSTERS |
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| MSDS |
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| SUPPLEMENTARY PROTOCOLS |
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| REFERENCES |
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| FREQUENTLY ASKED QUESTIONS |
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
Saliva DNA Isolation Kit |
45400 |
50 Preps |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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