Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Purification is based on spin column chromatography. Saliva DNA can be isolated from either saliva samples collected and preserved using Norgen's Saliva DNA Collection and Preservation Devices, or fresh saliva samples. Preserved saliva samples, or fresh saliva samples mixed with the provided Lysis Solution are first incubated for 1 hour. Next, Proteinase K and Binding Solution are then added to the sample, followed by the addition of ethanol. The resulting solution is loaded onto a spin-column (BIND). Under these conditions only the genomic DNA will bind to the column while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified genomic DNA is eluted into 200 µL of the provided Elution Buffer (ELUTE). Please see the procedure flowchart to the right.
Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
FEATURES AND BENEFITS
- Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 30 minutes.
- Sample collection is non-invasive and painless - Saliva offers an attractive alternative to blood or tissue for the isolation of human DNA samples for use in diagnostic applications.
- Compatible with Norgen's collection and preservation products - This product allows for the isolation of DNA from samples collected and preserved using Norgen's Saliva DNA Collection and Preservation Devices
- Recovered DNA is suitable for various downstream applications - Purified saliva DNA can be used in a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.