Sputum Liquification Buffer (10X)

(Cat# 28289)

Simple and efficient liquification of viscous sputum samples

Simple and efficient liquification
Sputum liquification to allow for the isolation of cells
Liquify sputum prior to nucleic acid isolation

Product Description
Product Description
Applications & Data
Applications & Data
Specifications
Specifications
Resources
Resources
Ordering
Ordering

Norgen’s Sputum Liquification Buffer is designed for the liquification of viscous sputum samples prior to isolation of cells for microscopy or nucleic acids for molecular testing. The homogenization of sputum samples is often desirable, particularly when there is a requirement for centrifugation to pellet cells or prior to the isolation of nucleic acids. The Sputum Liquification Buffer contains the mucolytic agent dithiothreitol which acts by reducing the disulfide bonds of the mucus proteins, thereby decreasing sample viscosity and allowing for easy handling and processing. Genomic DNA or RNA can then be isolated from the homogenized sample as Norgen’s Sputum Liquification Buffer is both DNAse and RNAse-free.

Norgen's Sputum Liquification Buffer (10X) is avalilable in 2 convenient sizes:

10 mL
100 mL (10 x 10 mL)

FEATURES AND BENEFITS

Facilitates homogenization of sputum samples – Allows even highly viscous samples to be processed easily and quickly
Convenient, pre-made solution – Save precious laboratory time by using premade buffer
Compatible with most DNA isolation methods - Nucleic acids can be isolated from the sputum samples via ethanol precipitation. Alternatively, nucleic acids can be isolated using other methods, such as Norgen's Sputum DNA Isolation Kit (Cat# 46200) which uses spin columns.

Download our brochure.

APPLICATIONS

The Sputum Liquification Buffer provides for an efficient liquification/homogenization of viscous sputum samples. The resulting sample can then be used for the subsequent purification of:

tumour cells
mycobacteria/bacteria
fungi and yeast
DNA
RNA

This reagent can be used with Norgen’s Sputum DNA Isolation Kit (Cat # 46200)

Figure 1. Isolation of Genomic DNA from Sputum Samples Liquefied using Norgen’s Sputum Liquification Buffer. Expectorated sputum samples (0.2 mL) were collected and an equal volume of Norgen’s Sputum Liquification Buffer (final concentration = 50 µg/mL dithiothreitol) was added to each sample. Samples were then incubated at 37°C with intermittent mixing to allow the samples to be completely homogenized. Genomic DNA was then isolated from the homogenized sputum samples using Norgen’s Sputum DNA Isolation kit (Cat# 46200) according to the kit protocol. For each 100 µL DNA elution, 10 µL was loaded into the wells of a 1X TAE, 1% agarose gel, run at 150 volts for 30 minutes. Gel was then visualized via ethidium bromide staining and photographed.

PROTOCOL

Sputum Liquification Buffer (10X) - Protocol
PDF version

PRODUCT INFORMATION SHEETS

APPLICATION NOTES

POSTERS

MSDS

SUPPLEMENTARY PROTOCOLS

REFERENCES

FREQUENTLY ASKED QUESTIONS