Plasma/Serum Exosome RNA Isolation Kit

Figure 1: Detection of Exosomal RNA Isolated from Different Plasma Volumes. Norgen’s Plasma/Serum Exosome RNA Isolation Kit was used to isolate plasma exosomal RNA from different plasma volumes ranging from 0.25 – 2mL Plasma. The purified plasma exosomal RNA was then used as the template in an RT-qPCR reaction to detect the human 5S gene. Three microlitres of the isolated urine exosomal RNA was used as the template in the RT step, and 3 μL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify the 5S gene from RNA isolated from different Plasma volumes (shown as different line colours), indicating the high quality of the isolated plasma exosomal RNA

Figure 2: Effective and Consistent Detection of Plasma Exosome RNA. Norgen's Plasma/Serum Exosome RNA Isolation Kit can effectively isolate RNA from plasma. Plasma Exosome RNA was isolated from 500 µL of human plasma prepared from blood collected on citrate, EDTA or Heparin in triplicates using Norgen's Plasma/Serum Exosome RNA Isolation Kit (blue), a ExoQuick Exosome Precipitation Reagent (green) and Qiagen’s QIAzol extraction followed by a modified Qiagen’s RNeasy Mini Kit cleanup (red). Stem loop RT-qPCR using primers specific to miR-21 and miR-16 as well as the housekeeping 5S rRNA was performed. In brief, three microliters of the 100 µL isolated RNA was then subjected to a 20 µL reverse transcription using 5S rRNA, miR-21 and miR-16 stem-loop reverse primer or reveres primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21, human miR-16 and the 5S rRNA. Norgen's Plasma/Serum Exosome RNA Isolation Kit is the only product that showed consistent detection of all tested transcripts with the highest quality regardless the type of the anti-coagulant used for blood collection.