Norgen’s Urine DNA Isolation Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL of urine. Many advantages favour the use of urinary DNA for cancer biomarker discovery and diagnosis of different pathogens over the use of blood and tissue samples. DNA found in urine can be divided into 2 basic categories. The larger genomic DNA (gDNA) species is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid. Both types of DNA can be isolated reliably using this kit. Typical yields of DNA isolated will vary depending on the input sample, with more concentrated samples tending to yield more DNA.
Norgen’s Urine DNA Isolation Maxi Kit (Slurry Format) can also be used for the isolation of viral DNA from urine. The viral DNA is isolated free from inhibitors and can be used directly as the template in a PCR reaction for viral DNA detection. The procedure can be used for the isolation of viral DNA from a broad range of DNA viruses.
Purification is based on using Norgen's proprietary resin as the separation matrix. Briefly, urine samples are mixed with Solution A, which contains our separation matrix. Next, samples are mixed with Solution B, which allows for lysis and binding to the resin (BIND). Next, the slurry is loaded onto a provided Mini Filter Spin Column, folllowed by washing of the bound DNA to remove the remaining proteins or other impurities (WASH). Lastly, the purified total urine DNA is eluted into the provided Elution Buffer (ELUTE). Please see the procedure flowchart to the right.
The kit allows for the removal of highly concentrated salts, metabolic wastes and proteins to allow for the isolation of high quality, concentrated DNA. Preparation time for a single sample is aprroximately 30 minutes. The purified urine DNA is compatible with molecular biological applications such as PCR, q-PCR and Southern Blot analysis.
FEATURES AND BENEFITS
- Fast processing - Rapid format allows for the processing of multiple samples in 45 minutes.
- Isolation of both types of urine DNA - Isolate both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation).
- High quality DNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated DNA to be used in various downstream applications.
- Recovered DNA is suitable for downstream applications - Purified urine DNA is compatible with molecular biological applications such as PCR, q-PCR and Southern Blot analysis.