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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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Yeast Genomic DNA Isolation Kit
(Cat# 18600, 26300)
For the rapid preparation of genomic DNA from yeast
- Rapid and convenient spin column format
- 96-well format available for high throughput
- High yields of genomic DNA
- High quality DNA for a wide range of downstream applications
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Norgen’s Yeast Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from a few cells up to 1 x 108 viable yeast cells (between 0.5 and 1 mL of culture). Purification is based on spin column chromatography using Norgen’s patented resin as the separation matrix.
Briefly, the yeast are pelleted and lysed, and the released genomic DNA is bound to Norgen's column in the presence of Binding Solution (BIND). Under these conditions only the genomic DNA will bind to Norgen's resin while most of the contaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNA is then washed to remove any remaining impurities (WASH). Lastly, the purified yeast genomic DNA is eluted into 100 - 200 µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right.
The Yeast Genomic DNA Isolation Kit allows for the isolation of genomic DNA from yeast and other cultured fungi. Typical purification yields will vary depending on the cell density of the yeast or fungal culture and species. Purified yeast genomic DNA can be used in a number of downstream applications including PCR and Southern blot analysis.
This kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
FEATURES AND BENEFITS
- Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 90 minutes, and the 96-well plates can also be processed in 90 minutes.
- High binding capacity of columns - The binding capacity of the columns in the Yeast Genomic DNA Isolation Kit is 25 µg.
- Isolate genomic DNA from yeast and other cultured fungus
- Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis.
- High quality DNA - No degradation of the genomic DNA isolated with the Yeast Genomic DNA Isolation Kit is observed.
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| APPLICATIONS
Purified yeast genomic DNA is highly suited to a wide range of applications including:
- Restriction enzyme digestions
- PCR
- Southern blot analysis.
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Figure 1. Isolation of High Quality Genomic DNA. Genomic DNA was isolated from 1 mL of overnight yeast culture (107 cells) using Norgen’s Yeast Genomic DNA Isolation Kit and a leading competitors kit. Ten microliters of the 200 µL purified DNA were run on a 0.9% agarose gel. Note the quality, consistency and yield of the DNA isolated using Norgen’s kit (Lanes 1 - 4) as compared to the DNA isolated using the competitor’s kit (Lanes 5 and 6). Lane M is Norgen’s MidRanger 1 kb DNA Ladder. The yield of the isolation is summarized in Figure 2.
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Figure 2. High Yield Purification. Genomic DNA was isolated from 1 mL of overnight yeast culture (107 cells) using Norgen’s Yeast Genomic DNA Isolation Kit and a competitors kit. Aside from the consistency and integrity of DNA isolated as shown in Figure 1, Norgen’s kit was found to have a significantly higher yield of DNA than the competitor’s kit. Quantification was performed using agarose gel densitometry. |
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Kit Specifications - Spin Columns
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Maximum Input
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1 x 108 yeast cells
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Average Yield*
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Up to 10 µg
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Column Binding Capacity
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25 µg
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Time to Complete 10 Purifications
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1.5 hours
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* Average yield will vary due to cell density of the yeast or fungal culture, the growth conditions and the species.
Yeast Genomic DNA Isolation Kit Contents - Spin Columns
1. Lysis Solution
2. Binding Solution
3. Proteinase K (lyophilized)
4. Wash Solution
5. Elution Buffer
6. Micro Spin Columns
7. Collection Tubes
8. Elution Tubes
9. Product Insert
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Kit Specifications - 96 Well Plates
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Binding Capacity Per Well
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10 mg
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Maximum Loading Volume Per Well
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500 mL
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Maximum Yeast Input per Well
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5 x 107 cells
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Time to Complete 96 Purifications
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2 hours
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Yeast Genomic DNA Isolation Kit Contents - 96-Well Kits
1. Lysis Solution
2. Binding Solution
3. Wash Solution
4. Elution Buffer
5. Proteinase K
6. 96-Well Filter Plate
7. Adhesive Tape
8. Collection Plates
9. Elution Plate
10. Product Insert
Storage Conditions and Product Stability
The Proteinase K should be stored at -20oC upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
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Order NOW at our online store:
Item |
Cat. No. |
Kit Size |
Buy Online |
Yeast Genomic DNA Isolation Kit |
18600 |
50 preps |
Buy Now |
Yeast Genomic DNA Isolation 96-Well Kit |
26300 |
192 preps |
Buy Now |
Order by Phone: Toll Free (USA & CANADA): 1-866-NORGENB (667-4362) or from anywhere at (905)227-8848.
Order by Email: order by sending an email to orders@norgenbiotek.com.
Order by Fax: Fax your order to (905)227-1061
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