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Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide.
As a testament of our commitment to quality, Norgen is proud to be an ISO 9001:2008 and ISO 13485:2003 registered company.
It is a pleasure to serve you and we look forward to working closely with you to provide the best sample preparation products for your specific needs. |
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Please contact us at:
info@norgenbiotek.com
Norgen Biotek Corp.
3430 Schmon Parkway,
Thorold
Ontario, Canada, L2V 4Y6
Tel: (905) 227-8848
Fax: (905) 227-1061 |
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- What If a variable speed centrifuge is not available?
• A fixed speed centrifuge can be used, however reduced yields may be observed.
- What will happen if my centrifugation speed varied from the recommended speed?
• This may lead to the degradation of the isolated nucleic acids or reduction in the total nucleic acid yields.
- At what temperature should I centrifuge my samples?
• All centrifugation steps are performed at room temperature. Centrifugation at 4°C will not adversely affect kit performance.
- Can I process a different plasma/serum volume?
• Yes, you can. All the buffers included in this kit are in a linear relationship to the volume of Plasma/Serum sample processed. Make sure that you do not deviate from the ratio specified in the product manual. The buffers are optimized per 1 mL of Plasma/Serum sample.
- What If I added more or less of the specified reagents’ volume?
• Adding less volume may reduce your RNA yields. Adding more may not affect the RNA yields EXCEPT if more Elution Buffer was added. Eluting your nucleic acids in higher volumes of Elution Buffer/solution will result in diluting your nucleic acids.
- What If I forgot to do a dry spin after my second wash?
• Your elution will be contaminated with the Wash Solution. This may dilute the nucleic acid yield in your elution and it may interfere with your down stream applications.
- Can I perform a second elution?
• Yes, you can. A second elution is possible, but it is recommended that this elution is performed in a smaller volume (50 µL).
- Why does my isolated RNA not perform well in downstream applications?
• If a different Elution Buffer was used other than the one provided in the kit, the buffer should be checked for any components that may interfere with the application. Common components that are known to interfere are high salts (including EDTA), detergents and other denaturants. Check the compatibility of your elution buffer with the intended use.
- Do I need to do a DNase treatment for my RNA Elution?
• You may need to do a DNase treatment to your isolated Plasma/Serum Circulating RNA. It is recommended that Norgen’s RNase-Free DNase I Kit (Cat# 25710) is used.
- Why is my A260/280 ratio low?
• This value may be low due to the nature of the RNA content in the plasma/serum sample. Plasma should not contain any cells, therefore no ribosomal RNA will be detected and hence the A260/280 ratio will be low. This low A260/280 ratio will not affect any downstream applications such as RT-qPCR or microarray analysis.
- What is the best miRNA to use for the validation of the efficiency of my miRNA isolation?
• If you wish to validate the efficiency of your miRNA isolation prior to use in downstream applications, we recommend the use of miRNA 16 as a reference for amplification.
- Why is the quality of my isolated RNA low?
• The RNA isolated from plasma or serum will be mainly a result of apoptosis, and will therefore be degraded. Usually the A260/A280 ratio for the RNA isolated from plasma or serum will be in the range of 1.4 - 1.6. This will not affect any downstream applications such as RT-PCR, RT-qPCR or microarray analysis..
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