E.coli O157:H7 TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz E.coli O157:H7 TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM41350 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for E.coli O157:H7 Available in TaqMan format for analysis E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk. TaqMan PCR Kit Click to expand options E.coli O157:H7 TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals (FAM and Cy5) above the baseline from FAM, Cy5 and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Positive (Cy5) result. Both PCR signals above the baseline from Cy5 and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR Positive (FAM) result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 4. Example of TaqMan PCR Negative result. No target DNA was detected in FAM and Cy5 channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 5. Example of TaqMan PCR inhibition result. No signal from FAM, Cy5 and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM41350 (100 preps) Cat. TM41310 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - E. coli O157:H7 Primer & Probe Mix 280 μL 280 μL E. coli O157:H7 Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM41350) E.coli O157:H7 TaqMan PCR Kit - Protocol (100 rxns) (TM41310) E. coli O157:H7 TaqMan Probe/Primer and Control Set - Protocol (100 rxns) SAFETY DATA SHEETS (TM41350) E.coli O157:H7 TaqMan PCR Kit - Safety Data Sheet Related Products Milk Bacterial DNA Isolation Kit Food DNA Isolation Kit
E.coli O157:H7 TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz E.coli O157:H7 TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM41350 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity