Saccharomyces cerevisiae TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saccharomyces cerevisiae TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM33350 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Saccharomyces cerevisiae Available in TaqMan format for analysis Saccharomyces cerevisiae plays a beneficial role in wine fermentation, in which it is the predominant species. Despite the beneficial role of S. cerevisiae in the food industry for food and beverage production, it is able to cause spoilage in wines. Uncontrolled yeast growth can alter the chemical composition of wine, detracting from its sensory properties of appearance, aroma, and flavour. If these faults are severe, the wine is rejected by consumers. S. cerevisiae contamination is found mainly in sweet wines, where fermentable sugar can support growth, and also in semidry bottled wines. Therefore a rapid and sensitive method to detect S. cerevisiae contamination is required to prevent wine spoilage. TaqMan PCR Kit Click to expand options Saccharomyces cerevisiae TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Saccharomyces cerevisiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM33350 (100 preps) Cat. TM33310 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Saccharomyces cerevisiae Primer & Probe Mix 280 μL 280 μL Saccharomyces cerevisiae Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM33350) Saccharomyces cerevisiae TaqMan PCR Kit - Protocol (100 rxns) (TM33310) Saccharomyces cerevisiae TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Related Products Fungi/Yeast Genomic DNA Isolation Kit
Saccharomyces cerevisiae TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Saccharomyces cerevisiae TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM33350 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity