Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol USD$279.00 SKU 29000 Format Spin Column Size 25 Preps Quantity Overview Details Documentation Citations Overview Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples Small urine and CSF input ranging from as low as 0.5 mL to 1 mL No phenol extractions Very sensitive and linear down to a few cells without the need for carrier RNA Bind and elute all RNA irrespective of size or GC content, without bias Rapid and convenient spin column protocol Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses. Details Supporting Data Previous Figure 1. Isolation and Detection of microRNA from Urine Samples. Norgen's Urine microRNA Purification Kit was used to isolate microRNA from 8 different 1.5 mL urine samples. The purified microRNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene. Five microlitres of the isolated RNA were used as the template in the RT step, and 5 µL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify the miR-21 gene in all cases, indicating the high quality of the isolated urine microRNA. The black line in the graph above corresponds to the No Template Control. Next Click for expanded view Kit Specifications Volume of Urine Processed 0.5 - 1 mL Size of RNA Purified All sizes, including < 200 nt Time to Complete 10 Purifications < 30 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 29000 (25 preps) Lysis Buffer A 2 x 20 mL Wash Solution A 18 mL Elution Solution A 6 mL Mini Spin Columns 25 Collection Tubes 25 Elution Tubes (1.7 mL) 25 Product Insert 1 Documentation PROTOCOLS (29000) Urine microRNA Purification Kit - Protocol (25 prep) APPLICATION NOTES Urine microRNA Purification Kit Effect of Microrna Gc Content On RNA Purification Efficiency - Application Note Effect of microRNA GC Content on RNA Purification Efficiency SAFETY DATA SHEETS (29000) Urine microRNA Purification Kit - Safety Data Sheet POSTERS Urine microRNA Purification Kit Differential Expression of Micrornas Assayed From Urine of Patients With Prostate Cancer Or Bph - Poster Effect of microRNA Content on RNA Purification Efficiency from Biological and Clinical Samples RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Title Analysis of microRNAs in Small Urinary Extracellular Vesicles and Their Potential Roles in Pathogenesis of Renal ANCA-Associated Vasculitis Journal Int. J. Mol. Sci.. 2022. Authors Jana Frydlova, Iveta Zednikova, Veronika Satrapova, Eva Pazourkova, Sarka Santorova, Zdenka Hruskova, Vladimir Tesar, Martin Vokurka, Petr Prikryl and Marie Korabecna Title Urinary Levels of miR-491-5p and miR-592 as Potential Diagnostic Biomarkers in Female Aging Patients with OAB: A Pilot Study Journal Metabolites. 2022. Authors Philippe G. Cammisotto, Abubakr H. Mossa, Samer Shamout and Lysanne Campeau Title Combined miRNA and SERS urine liquid biopsy for the point-of-care diagnosis and molecular stratification of bladder cancer Journal Molecular Medicine. 2022. Authors Tudor Moisoiu, Mihnea P. Dragomir, Stefania D. Iancu, Simon Schallenberg, Giovanni Birolo, Giulio Ferrero, Dan Burghelea, Andrei Stefancu, Ramona G. Cozan, Emilia Licarete, Alessandra Allione, Giuseppe Matullo, Gheorghita Iacob, Zoltán Bálint, Radu I. Badea, Alessio Naccarati, David Horst, Barbara Pardini, Nicolae Leopold & Florin Elec Title The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients Journal Cancer Medicine. 2022. Authors Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini Title Noninvasive Prognosis of Postmyocardial Infarction Using Urinary miRNA Ultratrace Detection Based on Single-Target DNA-Functionalized AuNPs Journal ACS Appl. Mater. Interfaces. 2018. Authors Qianlong Wang, Lancong Liu, Xiaoyi Chen, Tiantian Wang, Hua Zhou, Hui Huang, Linsen Qing, and Pei Luo Title MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study Journal PLOS | One. 2018. Authors Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby Title Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma Journal Anticancer Research. 2016. Authors Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O, Title Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs Journal Advances in Experimental Medicine and Biology. 2016. Authors Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M. Title MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection Journal Neoplasma. 2016. Authors Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M. Title Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy Journal Journal of Diabetes & Metabolism. 2016. Authors Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun Title Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens Journal ACS Appl. Mater. Interfaces. 2016. Authors Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F Title Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma Journal American Journal of Cancer Research. 2016. Authors Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer Journal Molecular Cancer. 2015. Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit Title MicroRNAs in urine are not biomarkers of multiple myeloma Journal Journal of Negative Results in Biomedicine. 2015. Authors Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková Title Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker. Journal BMC Cancer. 2015. Authors Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler. Title MicroRNA-21-5p upregulation in urine samples serves as novel biomarkers for early stage renal cell carcinoma patients diagnosis Journal Cancer Res. 2014. Authors Wei Meng, Hansjuerg Alder, Kirsteen Maclean, Simon Kirste, Petra Stegmaier, Anca Grosu, Arnab Chakravarti, Tim Lautenschlaeger Title Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus. Journal Chinese Journal of Nephrology. 2014. Authors Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L. Title Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients. Journal PLoS One. 2013. Authors Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T. Related Products Urine Total RNA Purification Maxi Kit (Slurry Format) Urine Cell-Free Circulating RNA Purification Mini Kit microRNA (cel-miR-39) Spike-In Kit
Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol USD$279.00 SKU 29000 Format Spin Column Size 25 Preps Quantity