Coconut tinangaja viroid (CTiVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Coconut tinangaja viroid (CTiVd) TaqMan RT-PCR Detection Kits SMART Save USD$749.00 USD$599.20 SKU TM50550 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for CTiVd Available in TaqMan format for analysis Coconut tinangaja viroid (CTiVd) is associated with tinangaja disease, a lethal disease of coconut palm which was first described in 1917 in Guam. This disease resembles coconut cadang-cadang disease, caused by a coconut cadang-cadang viroid (CCCVd), which occurs in the Philippines. The two viroids share about 65% overall sequence homology. Typical symptom of CTiVd include yellow spots on the leaves and the production of small, scarified, elongated nuts lacking kernels. TaqMan PCR Kit Click to expand options CTiVd TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents CTiVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM50550 (100 preps) Cat. TM50510 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - CTiVd Primer & Probe Mix 280 μL 280 μL CTiVd Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM50550) Coconut Tinangaja Viroid (CTiVd) TaqMan RT-PCR Kit - Protocol (100 rxns) Related Products Coconut Tinangaja Viroid (CTiVd) TaqMan Probe/Primer and Control Set Plant/Fungi Total RNA Purification Kit
Coconut tinangaja viroid (CTiVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Coconut tinangaja viroid (CTiVd) TaqMan RT-PCR Detection Kits SMART Save USD$749.00 USD$599.20 SKU TM50550 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity