Total Nucleic Acid Tubes
Get the swab that is perfect for your intended use
Swabs are sold separately, unless indicated otherwise
For Oral and Nasal Use
CY-93050
Oropharyngeal and Nasopharyngeal Flocked Swab
| Part No. | Head Material | Head Width |
| CY-93050 | Nylon Fiber | 20mm |
| Head Thickness | Head Length | Head Material |
| 1.3mm | 20mm | Polypropylene |
| Break Point | Total Length |
| 80mm | 150mm |
Certificates: CE Marked, FDA Approved
Sterility: Ethylene Oxide Sterilization
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For Oral Use
CY-98000
Oropharyngeal Swab
| Part No. | Head Material | Head Width |
| CY-98000 | Nylon Fiber | 20mm |
| Head Thickness | Head Length | Head Material |
| 1.3mm | 20mm | Polypropylene |
| Break Point | Total Length |
| 30mm | 150mm |
Certificates: CE Marked, FDA Approved
Sterility: Gamma Irradiation
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For Nasal Use
CM-FS913
Nasopharyngeal Swab
| Part No. | Head Material | Head Width |
| CM-FS913 | Nylon Fiber | 2.6mm |
| Head Thickness | Head Length | Handle Width |
| 3.0mm | 20mm | 2.5mm |
| Handle Material | Handle Length | Total Length |
| ABS | 127mm | 147mm |
| Packing | Applications | Break Point |
| 100/bag | Nasopharyngeal | 80mm |
Certificates: FDA Registered, CE-IVD, ISO9001, ISO 13485,
ISO 11137, GMP Compliant Facility
Sterility: Gamma Irradiation
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For Oral Use
901101
Oropharyngeal Swab
| Part No. | Head Material | Head Width |
| 901101 | Nylon Fiber | 50mm |
| Head Thickness | Head Length | Head Material |
| 1.3mm | 20mm | Polypropylene |
| Break Point | Total Length |
| 80mm | 154mm |
Certificates: CE Marked, FDA Approved
Sterility: Gamma Irradiation
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Total Nucleic Acid Preservation Tubes
- No need to immediately process samples
- Total DNA, including viral DNA, preserved at room temperature over 4 months
- Total RNA, including viral RNA, preserved at room temperature over 2 months
- Inactivate microorganisms including bacteria, fungi, yeast and viruses
- Inactivates SARS-CoV-2 virus - Explore the Application Note
- Ship swab samples at room temperature safely
- Compatible with most DNA and RNA isolation methods
- CE-IVD marked version available
Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months. Swab samples collected and preserved using Norgen’s Total Nucleic Acid Preservation Tubes are compatible with most commercially available DNA and RNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA and RNA isolation kits and reagents, including Norgen’s Total RNA Purification Kit (Cat# 17200), Norgen's Saliva/Swab RNA Purification Kit (Cat# 69100), and Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). For purification of total nucleic acids please refer to the supplementary protocol provided with Norgen’s Total RNA Purification Kit (Cat#17200).
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Ordering Information
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Get in touch with Norgen’s on site product specialists for more information or a direct quote for the product you are interested in.
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Specifications
|
Kit Specifications |
|
|
Stability of DNA at Room Temperature |
4 months |
| Stability of RNA at Room Temperature |
2 months |
Storage Conditions and Product Stability
The Total Nucleic Acid Preservation Tubes can be stored at room temperature for up 1 year without any reduction in kit performance. The collect before date is indicated on the tube label and the kit box label.
*Note: Swabs not included with Preservation Tubes.*
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Figure 1. Stability of RNA and DNA stored in Norgen's Total Nucleic Acid Preservative. GAPDH (for DNA) and β-actin (for RNA) were monitored over an 18 week period for DNA and 60 days for RNA from swab samples stored at ambient temperature in Norgens Total Nucleic Acid Preservation Tubes. RNA was isolated from 250 µL of preserved swab samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 using Norgens Total RNA Isolation Kit (Cat.# 17200). DNA was isolated from 250 µL of preserved swab samples on day 0 and 18 weeks using Norgens Saliva DNA Isolation Kit (Cat# Dx45400). Next, 4 µL of elution was used in a 20 L real-time PCR reaction using CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). RNA can be stored in Norgens Total Nucleic Acid Preservative for up to 60 days and DNA can be stored for up to 18 weeks at room temperature with no change in quality and quantity of RNA and DNA.
Figure 1. Stability of RNA and DNA stored in Norgen's Total Nucleic Acid Preservative. GAPDH (for DNA) and β-actin (for RNA) were monitored over an 18 week period for DNA and 60 days for RNA from swab samples stored at ambient temperature in Norgens Total Nucleic Acid Preservation Tubes. RNA was isolated from 250 µL of preserved swab samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 using Norgens Total RNA Isolation Kit (Cat.# 17200). DNA was isolated from 250 µL of preserved swab samples on day 0 and 18 weeks using Norgens Saliva DNA Isolation Kit (Cat# Dx45400). Next, 4 µL of elution was used in a 20 L real-time PCR reaction using CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). RNA can be stored in Norgens Total Nucleic Acid Preservative for up to 60 days and DNA can be stored for up to 18 weeks at room temperature with no change in quality and quantity of RNA and DNA.
Download full resolutionFigure 2. Stability of RNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the RNase Protein (RP) gene from 5 oropharyngeal swab samples and 5 nasopharyngeal swab samples purified using Norgens Total RNA Purification Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan One-Step RT-PCR.
Figure 2. Stability of RNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the RNase Protein (RP) gene from 5 oropharyngeal swab samples and 5 nasopharyngeal swab samples purified using Norgens Total RNA Purification Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan One-Step RT-PCR.
Download full resolutionFigure 3. Stability of DNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the GAPDH gene from 5 oropharyngeal swab samples and from 5 nasopharyngeal swab samples purified using Norgen's Saliva DNA Isolation Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan PCR.
Figure 3. Stability of DNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the GAPDH gene from 5 oropharyngeal swab samples and from 5 nasopharyngeal swab samples purified using Norgen's Saliva DNA Isolation Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan PCR.
Download full resolutionFigure 4. Inhibitory Effects of Norgens Total Nucleic Acid Preservative on the Growth of Bacteria, Yeast and Viruses. The effect of Norgens Total Nucleic Acid Preservative on the growth of microorganisms clearly demonstrated that Norgens preservative inhibits the growth of virus, bacteria and yeast, thereby resulting in non-infectious samples that can be handled and shipped safely.
Figure 4. Inhibitory Effects of Norgens Total Nucleic Acid Preservative on the Growth of Bacteria, Yeast and Viruses. The effect of Norgens Total Nucleic Acid Preservative on the growth of microorganisms clearly demonstrated that Norgens preservative inhibits the growth of virus, bacteria and yeast, thereby resulting in non-infectious samples that can be handled and shipped safely.
Download full resolutionFigure 5. Downstream Application of Preserved Swab RNA & DNA. The application of the RNA or DNA purified from preserved oropharyngeal swab samples was evaluated to detect RNase P (house keeping gene) and 16S rRNA (bacterial DNA) from swab samples collected from 60 donors and the preserved at room temperature overnight. Each individual swab sample contains different levels of cells, contributing to the variable Ct of each gene detection, but all passed the acceptance criteria for valid RT-PCR and PCR (< 35Ct, red line) in the 20 µL reaction, indicating that preserved swab RNA and DNA stored in Norgens Total Nucleic Acid Preservative is of high quality for downstream applications, such as real-time RT-PCR and PCR.
Figure 5. Downstream Application of Preserved Swab RNA & DNA. The application of the RNA or DNA purified from preserved oropharyngeal swab samples was evaluated to detect RNase P (house keeping gene) and 16S rRNA (bacterial DNA) from swab samples collected from 60 donors and the preserved at room temperature overnight. Each individual swab sample contains different levels of cells, contributing to the variable Ct of each gene detection, but all passed the acceptance criteria for valid RT-PCR and PCR (< 35Ct, red line) in the 20 µL reaction, indicating that preserved swab RNA and DNA stored in Norgens Total Nucleic Acid Preservative is of high quality for downstream applications, such as real-time RT-PCR and PCR.
Download full resolutionComplete Your Workflow With Sample Purification
Saliva/Swab RNA Purification Kit (Cat. 69100)
- Isolate high quality total RNA, including viral RNA, from fresh and preserved saliva and swab samples
- Fast and easy processing using rapid spin-column format
- Isolate total RNA, from large rRNA down to microRNA (miRNA)
- No phenol or chloroform extractions
- Very sensitive & linear down to a few cells/viral copies without the need for carrier RNA
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- CE-IVD marked version available
- Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix
| Cat. # | Name | Size | |
|---|---|---|---|
| Dx69100 | Saliva / Swab RNA Purification Kit Dx | 50 preps | View product page |
| 69100 | Saliva / Swab RNA Purification Kit | 50 preps | View product page |
| Dx69300 | Saliva/Swab RNA Purification 96-Well Kit Dx | 2 x 96-well plates | View product page |
| 69300 | Saliva/Swab RNA Purification 96-Well Kit | 2 x 96-well plates | View product page |