Purification

Norgen’s Cell-Free Circulating (CFC) DNA and RNA Purification Kits provide an efficient method for the purification of fragmented free-circulating DNA, RNA, exosomal RNA or total nucleic acids from variable volumes of plasma/serum or cell-free urine samples. The kits are able to isolate all sizes of circulating DNA or RNA irrespective of size or GC content, without bias. These kits provide an advantage over other available kits in that they do not require extension tubes for the purification of cfc DNA or RNA from large sample volumes. The purified cfc DNA or RNA are eluted in an elution solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern blot analysis, reverse transcription PCR, Northern blotting, RNase protection, primer extension and expression array assays.

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Characteristics

  • Isolate all sizes of cfc-DNA, cfc-RNA, and exosomal RNA, including microRNA
  • Various sample types: Plasma/Serum, Urine, CSF and other bodily fluids
  • Concentrate circulating DNA into a flexible elution volume ranging from (10 µL - 100 µL)
  • No phenol extractions nor carrier RNA
  • Inhibitor-free cfc-DNA and cfc-RNA
  • Fixative-free preservative, no cross-linking of DNA
  • Preserve cell-free DNA/circulating tumor DNA for 30 days at ambient temperature
  • No plasma volume loss after shipping/transportation

Norgen cfc-DNA Purification

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