cf-DNA/cf-RNA Preservative Tubes

The only tube that preserves & stabilizes both cell-free DNA and cell-free RNA.

Featured Publication: From Sampling to Sequencing: A Liquid Biopsy Pre-Analytic Workflow to Maximize Multi-Layer Genomic Information from a Single Tube Explore the findings

 

Product Description

Norgen's cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits enables the purification and concentration of inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen's cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.

Norgen’s cf-DNA/cf-RNA Preservative tubes are available for research use (Cat. 63950) and for in vitro diagnostic use (Cat. Dx63950). Cat. Dx63950 is not for sale in the US.

Performance
Norgen's cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen's cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.

Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.

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Features & Benefits

  • Unique chemistry that preserves and stabilizes both cf-DNA and cf-RNA in a single tube
  • Fixative-free preservative - no cross-linking of DNA and no plasma loss during transport
  • Stabilize cf-DNA/ct-DNA/cf-RNA for 30 days and CTCs for 14 days at ambient temperature
  • Stabilize cf-DNA/ct-DNA for 8 days at 37°C
  • Minimize costs with full transcriptomic and genomic profiling from one tube
  • High quality cf-DNA, ct-DNA, cf-RNA and exosomal RNA, compatible with a variety of downstream applications
  • Stabilized nucleic acids are chemically unmodified preventing bias in data analysis
  • Prevent hemolysis allowing better separation of high quality plasma with no genomic DNA contamination
  • Vacuumed PET (plastic) tubes to avoid breakage during shipping and transportation

Supporting Data

Ordering Information

Cat. No Product Add to Cart
Dx63950 cf-DNA/cf-RNA Preservative Tubes Dx
63950 cf-DNA/cf-RNA Preservative Tubes

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Next Generation Sequencing

Designed to Discover

Sequence Ultra-low Inputs from Plasma/Serum, Exosomes and More with Norgen's Small RNA-Seq Services.

  • Capture a Complete Range of Small RNA and miRNA species
  • Sequence Ultra-Low Inputs from Liquid Biopsies and Exosomes
  • Illumina® NextSeq Sequencing Platform
  • Understand Post-Transcriptional Regulation with Bioinformatics Analysis Service
  • Examine Differential Expression and Discover Novel Biomarkers
  • Fast Turn Around Time | 3 - 4 weeks
Next Generation Sequencing

Related Videos

Collection & Mixing Tutorial - cf-DNA/cf-RNA Preservative Tubes

DNA Purification Tutorial - Plasma/Serum Cell-Free Circulating DNA

RNA Purification Tutorial - Plasma/Serum RNA

Related Blogs

By: The Norgen Team

According to the World Health Organization, cancer was the leading cause of death worldwide in 2020 1. With millions of individuals dying from breast, lung, colorectal and other forms of cancers daily, researchers have dug deep into the world of circulating tumour DNA (ct-DNA) to learn more about early...

Select Publications

Featured
Title From Sampling to Sequencing: A Liquid Biopsy Pre-Analytic Workflow to Maximize Multi-Layer Genomic Information from a Single Tube
Journal Cancers. June 2021.
Authors Kendra K. Maass, Paulina S. Schad, Agnes M. E. Finster, Pitithat Puranachot, Fabian Rosing, Tatjana Wedig, Nathalie Schwarz, Natalie Stumpf, Stefan M. Pfister and Kristian W. Pajtler
Title Response to BRAF/MEK Inhibition in A598_T599insV BRAF Mutated Melanoma
Journal Case Reports in Oncology. 2019.
Authors Sara Bjursten, Christoffer Vannas, Stefan Filges, Florian Puls, Ankur Pandita, Henrik Fagman, Anders Ståhlberg, and Max Levin
Title Evaluation of Storage Tubes for Combined Analysis of Circulating Nucleic Acids in Liquid Biopsies
Journal International Journal of Molecular Sciences. 2019.
Authors Aoife Ward Gahlawat, Judith Lenhardt, Tania Witte, Denise Keitel, Anna Kaufhold, Kendra K Maass, Kristian W Pajtler, Christof Sohn, Sarah Schott
Title 554P KRAS-dependent and independent mechanisms of progressive disease (PD) in colorectal cancer (CRC) patients (pts) with liver metastases (LM) while monitoring on circulating cell free DNA (cfDNA)
Journal Annals of Oncology. October 23, 2018.
Authors A Kirov Z Mihaylova, V Petrova, T Todorov, D Petkova, A Garev, A Todorova-Georgieva
Documents Poster
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cf-DNA/cf-RNA Preservative Tubes are for the collection and the preservation of cf-DNA, circulating tumor DNA, and cf-RNA

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