Complete Stool Collection & Isolation Workflow Solutions

Stool Collection

Features & Benefits

Norgen's preservation system supports DNA stability for over 2 years & RNA stability for up to 7 days at ambient temperature. This reduces cold-chain dependence, shipping complexity, storage costs and sample degradation risk.

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Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples.

DNA and RNA were isolated from 200 µL of preserved stool from 4 different donors (lanes A to D) stored at room temperature at the indicated time points (DNA at 27 months and RNA at 7 days) using Norgen's Stool Nucleic Acid Isolation Kit (Cat# 45600). Next, 10 µL from the 75 µL eluted nucleic acid was loaded onto a gel for visual analysis. High integrity stool DNA was isolated from preserved stool samples that were stored at room temperature for 27 months, and high integrity RNA was isolated from stool samples stored for 7 days at ambient temperature. Lane M is Norgen's HighRanger 1 kb DNA Ladder (Cat. 11900).
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Figure 1. Norgen's Best-In-Class Preservation.

Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods.
Norgen's preservative inactivates viruses, prevents bacterial/fungal growth, reduces odor and Makes samples safer to transport and handle.

"We have been using Norgen's using the Stool Nucleic Acid Collection and Preservation Tubes and Stool Total RNA Purification Kit for many years for our projects focused on searching fecal biomarkers (small non-coding RNAs and bacterial species) to diagnose colorectal cancer non-invasively. The challenge was to collect stool samples and preserve nucleic acids in a way that patients could collect samples at home and bring to the receiving hospital without rush and preserving the samples. We successfully used the collection tubes for over 1000 samples in the frame of an European project and we were able to extract either RNA and microbial DNA for metagenome analysis."

— Barbara Pardini, Italian Institute for Genomic Medicine
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Figure 4. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions.

The microbial composition from the stool samples preserved in Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period while non-preserved stool samples showed a distance in the dendrogram from the original stool profile.

Stool Collection Products

CE Marked

Analyte

RNA (1)

DNA (1)

Total DNA (1)

Intended Use

- Any -

IVD (1)

RUO (4)

Sample Type

Human (2)

Shelf Life

2 Years (5)

Shipping Condition

Room Temperature (RT) (5)

Starting Material

Stool (5)

Storage Temperature

- Any -

Room Temperature (RT) (5)

Target Type

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Sample Available

Stool Nucleic Acid Collection and Preservation Tubes

SKU 45660 | 50 Tubes

A rapid and simple method for the collection, preservation and room temperature shipping of stool samples.

Fecal DNA Collection & Preservation Mini Tubes

SKU 27650 | 12 Tubes

A rapid and simple method for the preservation of DNA from fresh fecal samples

Stool Nucleic Acid Collection and Preservation Devices Dx

SKU Dx45660 | 50 Devices

For the collection and preservation of stool samples at ambient temperatures

Sample Available

Stool Nucleic Acid Collection and Preservation System

SKU 63700 | 50 Devices

All-in-one system for the rapid and simple collection, preservation, and room temperature storage and shipping of stool samples.

Sample Available

Fecal Swab Collection and Preservation System (Bulk Format)

SKU 45670-B | 50 Units

A rapid and simple method for the collection, preservation and shipping of stool samples

Coming Soon!
Stool EZCollect Tubes

Stool nucleic acid collection and preservation tubes with pre-loaded preservative, for a simplified collection process.
Interested? Contact us for early access!

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Stool Purification & Isolation

Features & Benefits

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Figure 1. Detection of bacterial and human genes from stool using qPCR.

DNA was isolated from 250 mg samples of stool using Norgen's Stool Nucleic Acid Isolation kit (Blue) and competitor Q’s Stool DNA kit (Red). Three different genes were targeted to assess sample purity: 16S rDNA (A), 18S rDNA (B) and GAPDH (C).

Figure 2. Higher Quality DNA than Competitor Z.

Stool DNA was isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit Dx and Competitor Z's Kit. DNA quality was then was measured by Nanodrop. Norgen's Stool DNA Isolation Kit Dx isolated DNA of the highest quality.
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Figure 1. Higher Yields of DNA than Competitor Q.

Stool DNA was isolated from 200 mg of fresh or preserved stool samples using Norgen’s Stool DNA Isolation Kit and Competitor Q's Kit. For evaluation, 10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel. Norgen's kit isolated much higher yields of DNA. *Stool was collected using Norgen’s Stool Nucleic Acid Collection and Preservation tubes (Cat. 45660). Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900).

Figure 1. High yields of stool total RNA were purified from 200 mg of human stool using Norgen's Stool RNA Purification Kit and a leading competitor's kit, in triplicate.

For analysis, 7.5 µL of each 75 µL elution was loaded on a 1.2 % 1x MOPS formaldehyde-agarose gel. Norgen's kit was found to have a higher yield of RNA, isolated from 200 mg of human infant stool.

"I used it recently for DNA isolation from cow stool samples, and I just have to say it was amazing. The kit was easy to use, and it had an amazing DNA yield, more than enough for the Illumina sequencing that I plan on conducting. I hope to use more of [Norgen] Biotek's kits in the future."

— Kalei Yoshimura
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Figure 7. High Quality DNA Isolated from Preserved Stool Samples.

DNA was isolated from 200 µL preserved stool samples using Norgen’s Stool DNA Isolation Kit (Magnetic Bead System).For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Stool samples were preserved in Norgen’s Stool Nucleic Acid Collection and Transport Tubes (Cat. 45630, 45660). M = Norgen’s HighRanger DNA Ladder (Cat. 11900).
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Figure 6. Distribution of 10 Different Fecal Microbiomes.

A) Principal Coordinate Analysis of 10 fecal microbiomes showing differences in the distribution of taxonomic classifications between samples up to class level. B) Hierarchical clustering of 10 fecal microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.

Stool Purification & Isolation Products

CE Marked

Analyte

Total RNA (2)

Multi-Analyte (1)

Total DNA (5)

Intended Use

- Any -

IVD (2)

RUO (6)

Sample Type

Human (2)

Shelf Life

2 Years (8)

Shipping Condition

Room Temperature (RT) (8)

Starting Material

Stool (8)

Storage Temperature

- Any -

Room Temperature (RT) (8)

Target Type

Sort by

Best Seller

Stool DNA Isolation Kit

SKU 27600 | Spin Column | 50 Preps

For the rapid and simple purification of microbial and host DNA from stool and fecal samples.

Stool DNA Isolation Kit Dx

SKU Dx27600 | Spin Column | 50 Preps

For the rapid and simple purification of bacterial and host DNA from stool and fecal samples for in vitro diagnostic use

Stool Total RNA Purification Kit Dx

SKU Dx49500 | Spin Column | 50 Preps

A convenient and rapid method to purify total RNA from small amounts of stool samples for in vitro diagnostic use

Sample Available

Stool Total RNA Purification Kit

SKU 49500 | Spin Column | 50 Preps

A convenient and rapid method to purify total RNA from small amounts of stool samples.

Stool Nucleic Acid Isolation Kit

SKU 45600 | Spin Column | 50 Preps

A convenient and rapid method to isolate total DNA and RNA from fresh, frozen and preserved stool samples.

Stool DNA Isolation Kit (Magnetic Bead System)

SKU 55700 | Magnetic Bead System | 50 Preps

For the rapid and simple purification of microbial and host DNA from stool and fecal samples.

Stool DNA Isolation 96-Well Kit (Magnetic Bead System)

SKU 63100 | High Throughput Magnetic Bead System | 2 x 96-Well Plates

For the rapid and simple purification of microbial and host DNA from stool and fecal samples.

Stool DNA Isolation 96-Well Kit

SKU 65600 | High Throughput | 2 x 96-Well Plates

For the rapid and simple purification of microbial and host DNA from stool and fecal samples.

FAQs

How should stool samples be collected for microbiome analysis?

Stool samples should be collected using standardized collection devices and preservation methods to minimize changes in microbial composition after collection. Proper collection and stabilization help ensure that the microbial profile measured in the laboratory accurately reflects the original sample.

What is the best way to preserve stool samples for DNA extraction?

The optimal preservation method depends on the study design and downstream applications. Preservation systems that stabilize nucleic acids at the time of collection can help maintain DNA integrity and reduce degradation during storage and transport.

Can RNA be extracted from stool samples?

Yes. Stool samples contain RNA from both host cells and microorganisms. Because RNA is highly susceptible to degradation, specialized preservation methods are often used to maintain RNA integrity for downstream applications such as RT-qPCR and RNA sequencing.

How long are stool samples stable after collection?

Sample stability depends on the collection device, preservation chemistry, storage conditions, and target analyte. Some preserved stool samples may remain stable for extended periods at room temperature, while others require refrigeration or freezing.

What biomarkers can be detected in stool samples?

Stool samples can contain microbial DNA, host DNA, RNA, proteins, metabolites, inflammatory markers, and other biological molecules. These biomarkers are commonly studied in microbiome, gastrointestinal, oncology, and infectious disease research.

What is the difference between 16S sequencing and shotgun metagenomics?

16S rRNA sequencing targets a specific bacterial gene to identify and compare bacterial populations within a sample. Shotgun metagenomic sequencing analyzes all genetic material present, providing broader information about microbial species, genes, and potential biological functions.

What factors affect stool microbiome results?

Microbiome results can be influenced by sample collection methods, preservation conditions, storage duration, transportation, DNA extraction protocols, sequencing methods, diet, medications, and individual biological variability.

What should researchers look for in a stool collection kit?

Researchers should consider sample stability, nucleic acid preservation, ease of use, transport requirements, compatibility with downstream workflows, regulatory requirements, and reproducibility when selecting a stool collection solution.

Can stool samples be shipped at room temperature?

Many modern stool preservation systems are designed to stabilize samples during ambient-temperature shipping. However, shipping requirements depend on the preservation technology, study objectives, and analytes of interest.

How does sample preservation affect microbiome sequencing?

Proper preservation helps maintain microbial composition and nucleic acid integrity between collection and analysis. Inadequate preservation may result in microbial overgrowth, nucleic acid degradation, or changes in community composition that can impact sequencing results.

Discover how Norgen automation solutions can help your laboratory operate more efficiently and stay ahead in a rapidly evolving scientific landscape.

Complete Stool Collection & Isolation Workflow Solutions