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Figure 1. Isolation of high quality RNA from HeLa cells stored in Norgens Total Nucleic Acid Preservative Tube at room temperature for up to 30 days. HeLa cells were stored in Norgens Total Nucleic Acid Preservative Tube (DR) and RNAlater (RL) for 30 days, and the RNA was isolated from the preserved samples using both an ethanol precipitation method and Norgens Total RNA Purification Kit (Cat. 17200). As it can be seen, the EtOH precipitation method failed to isolate RNA from the RNAlater preserved sample. High quality RNA was isolated from the samples preserved in Norgens Total Nucleic Acid Preservation Tubes using both isolation methods, indicating excellent preservation of the RNA and compatibility with various isolation methods.
| Cat. # | Description | Size | |
|---|---|---|---|
| Cat. 68800 | Swab Collection and Total Nucleic Acid Preservation System | 50 units | View product page |
Proceed to RNA isolation
Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation.
| Cat. # | Description | Size | |
|---|---|---|---|
| Cat. 17200, Cat. 37500, Cat. 17250, Cat. 17270 |
Total RNA Purification Kit | 50 Preps, 100 preps, 250 preps, 500 preps |
View product page |
| Cat. 24300, 24370 |
Total RNA Purification 96-Well Kit | 2 x 96-well plates, 6 x 96-well plates |
View product page |
| Cat. 24350, 24380 |
Total RNA Purification 96-Well Kit (Deep Well Format) | 2 x 96-well plates, 6 x 96-well plates |
View product page |
Proceed to PCR Based Detection
Figure 1. Analytical sensitivity for the detection of 2 SARS-CoV-2 nucleocapsid target genes and the RNase P internal Control target as recommended by the Centers for Disease Control and Prevention (CDC). Panel A represents the 2019-nCoV_N1 target, Panel B represents the 2019-nCoV_N2 target and Panel C represent the RNase P internal Control target. The analytical sensitivity of Norgens 2019-nCoV TaqMan RT-PCR Kit was determined by analyzing a dilution series of quantified Norgens 2019-nCoV RT-PCR Positive Control (Cat. PC67102) in triplicate (n=3) based on the use of TaqMan® technology. The linear range of Norgens 2019-nCoV TaqMan RT-PCR Kit has been determined to detect as low as 2 copies/µL with a confidence ≥ 90%.
| Cat. # | Description | Size | |
|---|---|---|---|
| Cat. TM67100 | 2019-nCoV TaqMan RT-PCR Kit | 50 rxns | View product page |
| Cat. TM67120 | 2019-nCoV TaqMan RT-PCR Kit | 500 rxns | View product page |
PCR Components
| Cat. TM67101 | 50 Rxns |
| Cat. TM67130 | 500 Rxns |
| 2019-nCoV_N1F | 2019-nCoV_N2F | RNAse P-F | |
|---|---|---|---|
| 2x108 | + | + | + |
| 2x107 | + | + | + |
| 2x106 | + | + | + |
| 2x105 | + | + | + |
| 2x104 | + | + | + |
| 2x103 | + | + | + |
| 2x102 | + | + | + |
| 2x101 | + | + | + |
| NTC | N/D | N/D | N/D |
| Cat.PC67102 | 50 µL |
| Cat.PC67120 | 500 µL |
| Cat. 28113 | 100 Rxns |
| Cat. 28114 | 200 Rxns |
| Cat. 28115 | 500 Rxns |
Figure 1. Performance of Norgens 2X One-Step RT-PCR Master Mix (28113) to detect SARS-CoV-2 using Norgens 2019-nCoV Primer & Probe Mixes (TM67101) and Norgens 2019-nCoV RT-PCR Positive Control (PC67102). The one-step RT-PCR reaction was prepared according to the CDC protocol and the sensitivity of detection of the three targets; 2019-nCoV_ N1 (Circle), 2019-nCoV_ N2 (Triangle) and RNase P (Cross), was compared with Competitor Ns product (Red).
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