Exosome Purification Kit

Figure 1. Purification and characterization of plasma-derived exosomes. Intact exosomes were purified from 1 mL human plasma using Norgen’s Exosome Purification Mini Kit (Cat# 75700). Purified exosomes were resuspended in 200 µL of Norgen’s ExoR buffer, diluted 1:1,000, and analyzed using the Particle Metrix ZetaView® Evolution instrument. Nanoparticle tracking analysis revealed a predominant population of plasma-derived exosomes with a mode particle size of 61 nm (A) and a total recovery of 2.58 × 10¹⁰ particles per mL of plasma (B). The isolated vesicles exhibited a clean size distribution with no detectable contaminating particles, indicating high purity. In addition, a broader population of exosomes ranging from 50–150 nm was consistently recovered (A), demonstrating efficient isolation across the expected exosomal size range.