Features and Benefits

Norgen’s EXTRAClean CleanAll DNA/RNA Clean-up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of DNA or RNA from different isolation methods or upstream applications. This kit purifies all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. It effectively removes PCR by-products including primers, dimers, enzymes, and unincorporated nucleotides. The purified DNA is fully compatible with restriction enzyme digestion, ligation, labeling, PCR and sequencing.

This kit also purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins and nucleotides, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays

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