Rapid purification of total RNA - including microRNA - from small input amounts
For research use only and NOT intended for in vitro diagnostics.
EXTRAClean Single Cell RNA Purification Kit
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Rapid purification of total RNA - including microRNA - from small input amounts
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Features and Benefits
- No phenol extractions
- Fast and easy processing of samples for the purification and the isolation of microRNA.
- Isolate total RNA, from large rRNA down to microRNA (miRNA) without compromising total yield
- More effective than any other method.
- EXTRAClean resin separation matrix provides highly purified RNA
Norgen’s EXTRAClean Single Cell RNA Purification Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, sorted cells, and micro dissected samples including laser-capture microdissection (LCM). The kit can recover RNA from as little as a single cell to 2 x 105 cells. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Details
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Kit Specifications
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| Maximum Column Binding Capacity | 10 μg |
| Maximum Column Loading Volume | 650 μL |
| Minimum Elution Volume | 8 μL |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
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Maximum Amount of Starting Material: Animal Cells Laser-Captured Microdissection (LCM) |
Single Cell to 2 x 105 cells Up to 2 x 105 cells |
| Time to Complete 10 Purifications | 20 minutes |
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Average Yield HeLa Cells (1 x 105 cells) HeLa Cells (100 cells) HeLa Cells (1 cell) |
~ 1.5 µg ~ 1.5 ng ≥ 1pg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 74900 (50 preps) |
|---|---|
| Buffer RL | 40 mL |
| Wash Solution A | 38 mL |
| Elution Solution A | 6 mL |
| EXTRAClean Single Cell RNA Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution tubes (1.7 mL) | 50 (2 x 25) |
| Product Insert | 1 |
Documentation
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