EXTRAClean Total RNA Purification Micro Kit

Features and Benefits

Fast and easy processing using rapid micro spin-column format
Small elution volume of 20 μL
Isolate total RNA, from large rRNA down to microRNA (miRNA) without compromising total yield
No phenol or chloroform extractions
Isolate high quality total RNA from a variety of sources
RNA can be isolated and detected from as little as a single animal cell

Norgen’s EXTRAClean Total RNA Purification Micro Kits provide a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, tissue samples, and micro dissected samples including laser-capture microdissection (LCM. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Kit Specifications
Maximum Column Binding Capacity	35 μg
Maximum Column Loading Volume	650 μL
Minimum Elution Volume	20 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM)	5 x 10⁵ cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 10⁵ cells
Time to Complete 10 Purifications	20 minutes
Average Yield HeLa Cells (1 x 10⁵ cells)	~1.5 μg

* for fibrous tissue, an additional Proteinase K treatment is required

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 74800 (50 preps)	Cat. 74850 (250 preps)
Buffer RL	40 mL	2 x 40 mL 1 x 30 mL
Wash Solution A	38 mL	3 x 38 mL
Elution Solution A	6 mL	1 x 20 mL
EXTRAClean Micro Spin Columns	50	250 (5 x 50)
Collection Tubes	50	250 (5 x 50)
Elution tubes (1.7 mL)	50 (2 x 25)	250 (10 x 25)
Product Insert	1	1

Documentation

Alternate formats available upon request by emailing info@norgenbiotek.com

PROTOCOLS

(74800) EXTRAClean Total RNA Purification Micro Kit - Protocol (50 Preps)

(74850) EXTRAClean Total RNA Purification Micro Kit - Protocol (250 Preps)

SAFETY DATA SHEETS

74800 - EXTRAClean Total RNA Purification Micro Kit - SDS

74850 - EXTRAClean Total RNA Purification Micro Kit - SDS