Overcoming Background Noise: Enhancing liquid-biopsy RNA Sequencing with EXTRAClean Technology

High-quality liquid biopsy NGS depends on obtaining sufficient target RNA while controlling background contamination and inhibitors before library preparation. Liquid biopsy matrices such as plasma, serum, urine and cerebrospinal fluid, as well as extracellular vesicles isolated from these biofluids, often contain very low amounts of cell-free RNA. In low-input samples, even trace exogenous DNA or RNA introduced by purification materials can be carried into library preparation and appear as background sequencing reads, while proteins, salts and other residual contaminants may reduce library conversion efficiency.

In this 45-minute technical session, Dr. Alex Chauhan reveals practical protocol strategies to eliminate background contamination and maximize nucleic acid recovery from challenging, low-input liquid samples. For high-throughput and clinical research labs, background noise means wasted reads and compromised sequencing budgets. Learn how combining contamination-free reagents with broad-spectrum RNA recovery, including microRNAs and small RNAs, can drastically reduce re-runs, boost library yield, and improve target representation.

Featuring head-to-head performance data on Norgen’s EXTRAClean™ manufacturing tech and silicon carbide-based extraction, this session delivers actionable insights to upgrade your lab’s data reliability and protect your downstream NGS investment.