Cell-free DNA (cf-DNA) and cell-free RNA (cf-RNA) are considered important biomarkers in plasma and serum for the detection of several diseases and conditions. These cf-DNA and cf- RNA are generally released in the bloodstream as the cells undergo apoptosis or necrosis. The cf-DNA and cf-RNA levels alter as per the underlying physiological processes, which might be driven by a disease or a condition. This is the basis of the non-invasive diagnosis and disease monitoring as well as disease prevention. The accurate detection of the cf-DNA/cf-RNA content in plasma/serum is influenced by the procedures implemented in the purification of cf-DNA/cf- RNA. In this webinar, we will discuss the different types of collection devices available to separate and preserve plasma/serum from blood. We will also discuss how different chemistries may affect cf-DNA/cf-RNA profiles. The webinar will also focus on cf-DNA/cf-RNA analysis methodologies and how these molecules can be exploited to generate data from instruments as simple as Qubit to as sophisticated as Next Generation Sequencing.