Phage DNA Isolation Kit For the rapid purification of total DNA from bacteriophages. For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Phage DNA Isolation Kit For the rapid purification of total DNA from bacteriophages. Price USD$269.00 SKU 46800 Format Spin Column Size 50 Preps 100 Preps Quantity Overview Details Documentation Citations Overview Isolate high quality DNA from a broad variety of phage strains High yields of total DNA Fast and easy processing using a rapid spin-column format No phenol or chloroform extractions or cesium chloride banding required High yields of DNA recovered 3-15 µg DNA from 106-1010 pfu/ mL of enriched phages This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more. Details Supporting Data Previous Figure 1. Effective Host Genomic DNA Removal without Reducing Phage DNA Yield. Total DNA was isolated from four enriched phage cultures using Norgen's Phage DNA Isolation Kit. A DNase I pre-treatment was performed prior to adding the provided Lysis Buffer. Briefly, 20 units of DNase I was added to 1 mL of enriched phage culture and the mixture was incubated at room temperature for 20 minutes. After the DNAase I treatment the procedure was followed. As a control, DNA was isolated from aliquots of the same 4 cultures using Norgen’s Phage DNA Isolation Kit without performing the DNase I treatment. For DNA analysis 10 µL of each 50 µL elution was loaded onto a 1X TAE agarose gel. As it can be seen, the phage DNA was safely protected from the DNase I treatment by its coat protein, while the host genomic DNA was efficiently degraded by the DNase I. Thus the DNase I pre-treatment resulted in less host gDNA contamination in the final phage elution without influencing the total phage DNA yield. Lane M is Norgen's Highranger 1 kb DNA Ladder (Cat. 11900) Figure 2. Optional Proteinase K Treatment Improves DNA Yield for Certain Phage Strains. Total DNA was isolated with and without the optional Proteinase K treatment using Norgen's Phage DNA Isolation Kit. Briefly, 4 µL of Proteinase K (20 mg/mL) was added to 1 mL of enriched phage culture and incubated at 55°C for 15 minutes with the phage Lysis Buffer. After the Proteinase K treatment the procedure was followed. As a control, DNA was isolated from aliquots of the same 8 cultures using Norgen's Phage DNA Isolation Kit without performing the Proteinase K treatment. For DNA analysis 10 µL of each 50 µL elution was loaded onto a 1X TAE agarose gel and the yield of DNA was compared from the eight different phage types (lane 1 to 8). As it can be seen, the optional treatment of Proteinase K improved the phage DNA yield in Lanes 2, 5 and 6 dramatically. Lane M is Norgen's Highranger 1 kb DNA Ladder (Cat. 11900) Next Click for expanded view Kit Specifications Column Binding Capacity 50 µg Maximum Column Loading Volume 650 µL Size of DNA Purified All sizes Maximum Amount of Starting Material 1 x 1010 pfu/mL enriched phages Average Yield* 3-15 µg DNA from 106-1010 pfu/mL of enriched phages Time to Complete 10 Purifications 45 minutes * Average yields will vary depending upon a number conditions used and developmental stage. Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 46800 (50 preps) Cat. 46850 (100 preps) Lysis Buffer B 40 mL 2 x 40 mL Wash Solution A 38 mL 2 x 38 mL Elution Buffer B 8 mL 2 x 8 mL Spin Columns 50 100 Collection Tubes 50 100 Elution Tubes (1.7 mL) 50 100 Product Insert 1 1 Documentation PROTOCOLS (46800) Phage DNA Isolation Kit - Protocol (50 prep) (46850) Phage DNA Isolation Kit - Protocol (100 prep) SAFETY DATA SHEETS (46800, 46850) Phage DNA Isolation Kit - Safety Data Sheet FLYERS Flyer Citations Powered by Bioz See more details on Bioz Related Products Bacterial Genomic DNA Isolation Kit RNase-Free DNase I Kit Soil DNA Isolation Kit
Phage DNA Isolation Kit For the rapid purification of total DNA from bacteriophages. For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Phage DNA Isolation Kit For the rapid purification of total DNA from bacteriophages. Price USD$269.00 SKU 46800 Format Spin Column Size 50 Preps 100 Preps Quantity
