Ideal for PCR product size determination
For research use only and NOT intended for in vitro diagnostics.
Norgen Biotek products are not subject to any U.S. tariffs.
Details
Supporting Data
Figure 1. 50 bp DNA Ladder.
50 bp DNA Ladder (Cat# 13525) - 5 x 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
500
|
79
|
|
2
|
450
|
67
|
|
3
|
400 |
59
|
|
4
|
350 |
55
|
|
5
|
300 |
49
|
|
6
|
250 |
76
|
|
7
|
200 |
33
|
|
8
|
150 |
22
|
|
9
|
100
|
31
|
|
10
|
50 |
29
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.