For Whole Blood Sample Collection and Preservation of Cell-Free Circulating DNA

  • Fixative-free preservative, no cross-linking of DNA
  • Preserve cell-free DNA/circulating tumor DNA for 30 days at ambient temperature
  • No plasma volume loss after shipping/transportation
  • Prevent hemolysis allowing better separation of plasma
  • Prevent apoptosis of blood cells and fragmentation of genomic DNA
  • Produce high quality/quantity of plasma cf-DNA/ct-DNA
  • Vacuumed to draw 8.4 mL of blood in 10 mL tubes
  • PET tubes to avoid breakage during shipping & handling
  • For maximum plasma cf-DNA yield and recovery, process the entire plasma volume recovered from Norgen's cf-DNA Preservative Tube
  • The highest plasma cf-DNA quantity and quality can be obtained by combining both Norgen's cf-DNA Preservative Tubes and Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits (55600 and 55800).

ItemsCat. #Size
cf-DNA Preservative Tubes 63950 50 Tubes
cf-DNA Preservative Tubes 63960 100 Tubes

cf-DNA Preservative Tubes

Norgen's cf-DNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cell-free circulating DNA/circulating tumor DNA in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum Circulating DNA Purification Kits (Slurry Format) enables the purification and concentration of an inhibitor-free cell-free circulating DNA/circulating tumor DNA in a very small elution volume that is compatible with any downstream application.

This product is for research use only and not for use in diagnostic procedures.

Performance

Norgen's cf-DNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cell-free circulating DNA. The cf-DNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA into plasma (see Figure 4). Moreover, the cell-free circulating DNA/circulating tumor DNA levels are stable for up to 30 days at room temperature or up to 8 days at 37°C (Figure 1 and Figure 5). Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma.

Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA Preservative Tubes and purified using Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum Circulating DNA Purification Kits (Slurry Format) yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA for any downstream application assays, including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis, microarrays and NGS.

Workflow

Collection and Mixing Instructions

NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950

Kit Specifications
Tube Size
16 x 100 mm
Tube Type PET Tube
Blood Draw Volume
8.4 mL in 10 mL Tubes
Preservative Volume
1.6 mL
Recovered Plasma Volume Approximately 6-7 mL
(not affected by shipping)
Anticoagulant
Proprietary
Preservative
Non formaldehyde - Proprietary preservation agent
Length of Preservation
Up to 30 days at room temperature (15-25°C)
Up to 8 days at 37°C

Storage Conditions and Product Stability

  1. When stored at room temperature (15-25°C), unfilled Norgen cf-DNA Preservative Tubes are stable through the expiration date. See tube label for expiry date.
  2. Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA Preservative Tubes.
  3. Do not freeze unfilled Norgen cf-DNA Preservative Tubes for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
  4. Blood samples collected in Norgen's cf-DNA Preservative Tubes are stable for up to 30 days when stored at room temperature (15-25°C)
  5. Blood samples collected in Norgen's cf-DNA Preservative Tubes are stable for up to 8 days when stored at 37ºC


Kit Components
Component Cat. 63950 (50 tubes) Cat. 63960 (100 tubes)
cf-DNA Preservative Tubes 50 100
Product Insert 1 1