Purchasing Information


  • CE-IVD marked in accordance with EU Directive 98/79/EC
  • Multiplex RT-PCR system detects two SARS-CoV-2 specific genes (N gene and ORF1ab) in a single one step RT-PCR reaction
  • Primer & Probe Mixes contain all N gene, ORF1ab and human RP assays
  • Positive Control contains the three SARS-related target genes: N gene, ORF1ab gene as well as the human RP (internal control).
  • Convenient ready-to-use  2X One-Step RT-PCR Master Mix
  • Find out more information about our complete COVID-19 workflow
 

CE marked version is not available for sale in the US and Canada

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COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx (Cat. DxTM67300) 500 rxns

  • CE-IVD marked in accordance with EU Directive 98/79/EC
  • Multiplex RT-PCR system detects two SARS-CoV-2 specific genes (N gene and ORF1ab) in a single one step RT-PCR reaction
  • Primer & Probe Mixes contain all N gene, ORF1ab and human RP assays
  • Positive Control contains the three SARS-related target genes: N gene, ORF1ab gene as well as the human RP (internal control).
  • Convenient ready-to-use  2X One-Step RT-PCR Master Mix
  • Find out more information about our complete COVID-19 workflow
 

Click for expanded view


COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx

Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains three SARS-CoV-2 detection targets; N (Nucleoprotein), ORF1ab (Open reading frame1ab) and the human RNase P transcript (RP - HEX/VIC) as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/ORF1ab/RP Positive Control contains an in vitro RNA transcript for the two SARS-related target genes: N gene, ORF1ab gene as well as the human RP gene (internal control).

Positive results are indicative of SARS-CoV-2 RNA detection, however clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. Positive results do not rule out co-infection with other viruses and therefore the agent detected may not be the definite cause of disease. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Any negative results must be combined with clinical observations, patient history, and epidemiological information.

Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx is intended for use by professional users including clinical laboratory personnel experienced and trained in molecular biology techniques including real-time PCR and in vitro diagnostic procedures.

Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx was developed and validated to be used with the BioRad CFX96 TouchTM Real-Time PCR Detection System.

 

Storage Conditions and Product Stability

  • All kit components should be stored at -20°C upon arrival
  • Repeated thawing and freezing (> 3x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
  • All reagents can be stored for 6 months at -20°C without showing any reduction in performance.


Want a discount?

Create your account to receive 15% off on your first purchase!

Full Kits

COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx (Cat. DxTM67300) 500 rxns

  • CE-IVD marked in accordance with EU Directive 98/79/EC
  • Multiplex RT-PCR system detects two SARS-CoV-2 specific genes (N gene and ORF1ab) in a single one step RT-PCR reaction
  • Primer & Probe Mixes contain all N gene, ORF1ab and human RP assays
  • Positive Control contains the three SARS-related target genes: N gene, ORF1ab gene as well as the human RP (internal control).
  • Convenient ready-to-use  2X One-Step RT-PCR Master Mix
  • Find out more information about our complete COVID-19 workflow
 

Click for expanded view


COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx

Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains three SARS-CoV-2 detection targets; N (Nucleoprotein), ORF1ab (Open reading frame1ab) and the human RNase P transcript (RP - HEX/VIC) as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/ORF1ab/RP Positive Control contains an in vitro RNA transcript for the two SARS-related target genes: N gene, ORF1ab gene as well as the human RP gene (internal control).

Positive results are indicative of SARS-CoV-2 RNA detection, however clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. Positive results do not rule out co-infection with other viruses and therefore the agent detected may not be the definite cause of disease. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Any negative results must be combined with clinical observations, patient history, and epidemiological information.

Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx is intended for use by professional users including clinical laboratory personnel experienced and trained in molecular biology techniques including real-time PCR and in vitro diagnostic procedures.

Norgen’s COVID-19 TaqMan RT-PCR Kit (N/ORF1ab genes) Dx was developed and validated to be used with the BioRad CFX96 TouchTM Real-Time PCR Detection System.

 

Storage Conditions and Product Stability

  • All kit components should be stored at -20°C upon arrival
  • Repeated thawing and freezing (> 3x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
  • All reagents can be stored for 6 months at -20°C without showing any reduction in performance.

 

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