EXTRAClean microRNA Purification Kit
For the rapid purification of microRNA without phenol
For research use only and NOT intended for in vitro diagnostics.
EXTRAClean microRNA Purification Kit
Best & First In Class!
Enhance your sequencing reads.
For the rapid purification of microRNA without phenol
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Features and Benefits
- No phenol extractions
- Fast and easy processing of samples for the purification and the isolation of microRNA.
- No time-consuming ultracentrifugation.
- More effective than any other method.
- EXTRAClean resin separation matrix provides highly purified RNA
Norgen’s EXTRAClean microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants and blood. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s EXTRAClean microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, northern blotting and microarray analysis.
Details
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Kit Specifications
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| Maximum Column Binding Capacity | Up to 50 μg RNA |
| Maximum Column Loading Volume | 650 μL |
| Minimum Elution Volume | 20 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material: Animal Cells Animal Tissues Bacteria Plant Tissues Blood |
3 x 106 cells 5-25 mg 1 x 109 cells 50 mg 100 μL |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 75000 (25 preps) |
|---|---|
| Buffer RL | 40 mL |
| Wash Solution A | 38 mL |
| Elution Solution A | 6 mL |
| EXTRAClean Large RNA Removal Column | 25 |
| EXTRAClean microRNA Enrichment Column | 25 |
| Collection Tube | 50 |
| Elution tubes (1.7 mL) | 50 (2 x 25) |
| Product Insert | 1 |
Documentation
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