EXTRAClean Oligo Clean-Up and Concentration Kit

Features and Benefits

Norgen’s Oligo Clean-Up and Concentration Kit provides a rapid, simple and efficient procedure for the purification and concentration of up to 10 µg of oligonucleotides. The kit is used for the purification of synthesized oligonucleotides, as well as the clean-up of oligonucleotides from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling. Single or double stranded DNA or RNA oligonucleotides larger than 10 bp can be purified with the kit, therefore the kit is not recommended for the removal of PCR primers. The kit purifies oligonucleotides from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction. The kit provides a high quality product with up to 90% recovery. The purified oligonucleotides can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, in situ hybridization, and RNAi studies.

The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS.

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