EXTRAClean Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Kits

Features and Benefits

Norgen’s EXTRAClean Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit provides a fast, reliable reproducible and simple procedure for isolating total circulating Nucleic Acid (DNA and RNA) from various amounts of plasma/serum inputs, with various kit formats address different plasma/serum input volumes. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of cfc-DNA and circulating RNA, including microRNA, as well as all sizes of exosomal RNA. Norgen’s EXTRAClean Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit provide a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. Nucleic Acids can be isolated from either fresh or frozen samples using this kit.

Background

Cell-free circulating RNA, including exosomal RNA in plasma or serum, has the potential to provide biomarkers for certain cancers and disease states, and includes tumor-specific extracellular RNA in the blood. Exosomes are 40 - 100 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which depend upon the tumour cell type from which they are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses, they can be efficiently recovered from biological fluids, such as plasma or serum.

EXTRAClean Plasma/Serum RNA Purification Mini Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL.

EXTRAClean Plasma/Serum RNA Purification Midi Kit

This utilizes a two-column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

EXTRAClean Plasma/Serum RNA Purification Maxi Kit

This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Details

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