Features and Benefits

Norgen’s cfc-DNA/cfc-RNA Advanced Fractionation Kit provides fast, reliable and simple procedures for the fractionation of the highest quality and t quantity of all sizes of circulating DNA and circulating RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL into two separate elutions. Purification is based on using Norgen’s proprietary resin separation matrix. The kits are designed to isolate total cfc nucleic acid from either fresh, preserved or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-RNA and cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum total cfc-nucleic acid is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS. transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS.

This kit can purify RNA/DNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 0.5 mL to 6 mL. The purified plasma/serum RNA is eluted in a flexible final volume of 25 µL to 50 µL.

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