Features and Benefits

Norgen’s EXTRAClean Plasma/Serum RNA/DNA Purification Mini Kit provides a fast, reliable reproducible and simple procedure for the sequential purification of circulating/exosomal RNA and cell-free circulating DNA (cfc DNA) from a single plasma/serum sample. Norgen’s EXTRAClean Plasma/Serum RNA/DNA Purification Mini Kit can purify RNA/DNA from various amounts of plasma/serum ranging from 10 μL to 200 μL. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA (including microRNA), all sizes of exosomal RNA, and all sizes of cfc DNA. Norgen’s EXTRAClean Plasma/Serum RNA/DNA Purification Mini Kit provides a clear advantage over other available kits in that it does not require phenol/chloroform or any protease treatments. RNA/DNA can be isolated from either fresh or frozen samples using this kit, and the kit allows the user to elute into a flexible elution volume ranging from 10 µL to 25 µL. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS.

Background

Typical yields of free-circulating, exosomal RNA and cfc-DNA vary depending on the input sample, as the amount of RNA/DNA present in plasma and serum will vary depending upon the health status of the individual. Normally, the RNA/NA yield from plasma or serum is highly variable (ranging from 1 to 100 ng/mL). Variability is also observed between samples collected from the same donor at different times during the day. This kit is suitable for the isolation of RNA and DNA from serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Details

Documentation