For rapid and simple combined purification of all sizes of cell-free circulating DNA (cfc-DNA), and circulating tumour DNA (ct-DNA) and circulating/exosomal RNA, including microRNA
For research use only and NOT intended for in vitro diagnostics.
EXTRAClean Plasma/Serum Total cfc-Nucleic Acid Advanced Purification Kit
Best & First In Class!
Enhance your sequencing reads.
For rapid and simple combined purification of all sizes of cell-free circulating DNA (cfc-DNA), and circulating tumour DNA (ct-DNA) and circulating/exosomal RNA, including microRNA
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Features and Benefits
- Versatile plasma/serum input ranges
- No phenol extractions
- Fast and easy processing of samples for the purification and the isolation of Total cfc-Nucleic Acid
- No time-consuming ultracentrifugation
- More effective than any other method
- EXTRAClean resin separation matrix provides highly purified Total cfc-Nucleic Acid
Norgen’s Plasma/Serum Total cfc-Nucleic Acid Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of all sizes of circulating DNA and circulating RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL combined into one elution. Purification is based on using Norgen’s proprietary resin separation matrix. The kits are designed to isolate total cfc-nucleic acid from either fresh, preserved or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-RNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum total cfc-nucleic acid is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
This kit can purify cfc-DNA/cfc-RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 0.5 mL to 6 mL. The purified plasma/serum Total cfc-Nucleic Acid is eluted in a flexible final volume of 25 µL to 50 µL.
Details
| Kit Specifications | |
| Minimum Plasma/Serum Input | 1 mL |
| Maximum Plasma/Serum Input | 6 mL |
| Size of cfc-RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Optimal size of purified cfc-DNA | 146-180 bp |
| Elution Volume | 25-50 μL |
| Time to Complete 10 purifications | 45 minutes |
| Average Yields¥ | Variable depending on specimen |
Â¥ Please check Appendix A in the protocol for Average Plasma/Serum Yields and Common Total Nucleic acid Quantification Methods.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
| Component | Cat. 74100 (50 preps) |
|---|---|
| Binding Buffer A | 20 mL |
| Proteinase K in Storage Buffer | 6.25 mL |
| Slurry E | 12.5 mL |
| Lysis Buffer A | 130 mL |
| Wash Solution A | 38 mL |
| Elution Buffer C | 2 x 8 mL |
| Elution Buffer F | 15 mL |
| Elution Solution A | 20 mL |
| EXTRAClean Micro Spin Columns | 100 |
| EXTRAClean Mini Filter Spin Columns | 50 |
| Collection Tubes | 100 (2 x 50) |
| Elution tubes (1.7 mL) | 100 (4 x 25) |
| Product Insert | 1 |
Documentation
- in a timely manner that considers the person's accessibility needs due to disability; and
- at a cost that is no more than the regular cost charged to other persons