EXTRAClean Total RNA Purification Plus Micro Kit

Features and Benefits

Norgen’s EXTRAClean Total RNA Purification Micro Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, tissue samples, and microdissected samples including laser-capture microdissection (LCM).The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Background

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). The DNA is then captured on a EXTRAClean Genomic DNA Removal Column. Ethanol is then added to the flowthrough of the DNA removal step, and the solution is loaded onto an EXTRAClean RNA Purification Micro Column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus, only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with Elution Solution A. The special design of the micro spin-column allows a small elution volume of as little as 20 L. The purified RNA is of the highest integrity and can be used in a number of downstream applications.

Details

Documentation