EXTRAClean Urine Exosome and Free-Circulating RNA Isolation Kit
For the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different urine sample volumes up to 30 mL
For research use only and NOT intended for in vitro diagnostics.
EXTRAClean Urine Exosome and Free-Circulating RNA Isolation Kit
Best & First In Class!
Enhance your sequencing reads.
For the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different urine sample volumes up to 30 mL
Overview
- Ensure optimal results for sensitive applications like NGS
- Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
- Versatile sample input ranges.
- The purified exosomal RNA is free from any circulating RNA-binding proteins.
- No phenol extractions, Proteinase K treatment nor carrier RNA required.
- No time-consuming ultracentrifugation, filtration nor special syringes are required.
- No precipitation reagents nor overnight incubation required.
- Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 μL to 100 μL.
- Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.
Norgen’s EXTRAClean Urine Exosome and Free-Circulating RNA Isolation Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different urine sample volumes ranging from 250 μL to 30 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Details
Supporting Data
Kit Specifications (Spin Column)
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Minimum Urine Input | 2 mL |
Maximum Urine Input | 10 mL |
Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
Elution Volume | 50-100 μLL |
Time to Complete 10 Purifications | 35-40 minutes |
Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Documentation
(73510) EXTRAClean Urine Exosome and Free-Circulating RNA Isolation Midi Kit (Spin Column) - Protocol (25 preps)
(73520) EXTRAClean Urine Exosome and Free-Circulating RNA Isolation Maxi Kit (Spin Column) - Protocol (15 preps)