Name: EZCollect Saliva DNA Device
Brand: Norgen Biotek Corp.
SKU: RU69900
Price: 16 USD
Availability: InStock

Features and Benefits

Collect 1.4mL saliva
Saliva DNA stable for 6 years at room temperature
Easy at home use

Norgen’s EZCollect Saliva DNA Device RU is designed for:

Simple and non-invasive home-based saliva collection
Preservation of DNA in saliva samples at ambient temperature. Intended for Research Use Only

Collection Protocol

Deposit saliva into the Collection Tube up to the indicated line.

If you notice excess bubbles, eliminate them by gently tapping the tube on a hard surface.
Remove the Collection Funnel. Cap the Collection Tube by pushing down and twisting until secured, and the Yellow Saliva DNA Preservative will be released.

Discard or recycle the Collection Funnel.
Shake well for 10 seconds to mix the saliva with the Yellow Saliva DNA Preservative

The saliva sample is preserved and pathogens & viruses, including SARS-CoV-2, are now inactivated.
The Collection Tube, containing the saliva sample is ready for transport.

Details

Supporting Data

Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for over 2 Years. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 24 months. Saliva DNA was subsequently isolated at 4 months, 8 months, 16 months and 24 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 24 months at ambient temperatures in Norgen's Saliva DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 24 month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).

Figure 2. GAPDH Real Time PCR Amplification of Saliva DNA from Saliva Samples Stored at Room Temperature from Day 0 up to 24 Months. All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition and difference in Ct. Red: Day 0, Blue: 4 months, Purple: 8 months, Green: 16 months and Sky blue: 24 months. Black: No template control.

Figure 3. V3-V4 16s rRNA PCR amplification for Illumina MiSeq 16s rRNA library preparation. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 2 years at room temperature in Norgen’s Saliva DNA Preservative. The PCR result shows the clean PCR amplification at ~550 bp indicating the right target of V3-V4 16s rRNA , with no difference between day 0 and 24 months. M: FastRunner DNA Ladder (Cat. 12800).

Storage Conditions and Product Stability
This device should be stored at room temperature for up to 2 years without any reduction in performance prior to Saliva Sample Collection. After the Saliva Sample Collection, the saliva DNA in preserved samples is stable for up to 6 years at room temperature (15-30°C).