For research use only and NOT intended for in vitro diagnostics.

A ready-to-use system for the isolation and detection of Feline Herpes Virus using end-point PCR

  • Rapid isolation of high quality DNA from plasma/serum, swabs or viral culture
  • Contains two ready-to-use 2X PCR Master Mixes
  • High sensitivity and specificity
  • Includes an isolation control and a PCR control
  • Primer set and controls also available separately
  • Ideal for use in:
    1. Surveillance of Drug Resistant Pathogens
    2. Epidemiologial Studies
    3. Field Surveillance of Pathogens
    4. Surveys

ItemsCat. #Size
Feline Herpes Virus PCR Detection Kit 44300 24 Preps
Feline Herpes Virus PCR Primer Set and Controls 44310 100 Rxns

Feline Herpes Virus PCR Detection Kit

Norgen’s Feline Herpes Virus PCR Detection Kit is a ready-to-use system for the isolation and detection of FHV from plasma/serum samples, swabs and viral culture. First, the kit contains components for the rapid isolation of total DNA, including viral DNA, from the samples using spin-column chromatography based on Norgen’s proprietary resin. Second, the kit contains FHV Master Mix to allow for PCR amplification, as well as a Control Master Mix to allow for amplification of both an isolation control and a PCR control. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves.

The FHV PCR Detection Mastermix contains reagents and enzymes for the specific amplification of a 338 bp region of the viral genome. In addition, Norgen’s FHV PCR Detection Kit contains a second Mastermix, the PCR Control Master Mix, which can be used to identify possible PCR inhibition and/or inadequate isolation via a separate PCR reaction with the use of the provided PCR control (PCRC) or Isolation Control (IsoC), respectively. This kit is designed to allow for the testing of 24 samples. The FHV PCR Primer Set and Controls are also available separately for end-point PCR detection.

Linear Range

  • The linear range of Norgen’s FHV PCR Detection Kit was determined by analysing a dilution series of a FHV quantification standards ranging from 100 ag to 1 pg.
  • Each dilution has been tested in replicates (n = 4) using Norgen’s FHV PCR Detection Kit on a 1X TAE 1.7% agarose gel.
  • The linear range of Norgen’s FHV PCR Detection Kit has been determined to cover concentrations from 100 ag to 1 ng
  • Under the conditions of the Norgen’s FHV DNA Isolation procedure, Norgen’s FHV PCR Detection Kit covers a linear range from 100 copies to 1 x 106 copies.

Norgen’s Feline Herpes Virus PCR Detection Kit Contents
Binding Solution 1
Binding Solution 2
Lysis Solution
Wash Solution
Elution Buffer
Mini Filter Spin Columns
Collection Tubes
Elution tubes (1.7 mL)
2x FHV Detection PCR Master Mix
2x PCR Control Master Mix
Isolation Control (IsoC)
FHV Positive Control (PosC)
Nuclease Free-Water
Norgen’s DNA Marker
Product Insert

Feline Herpes Virus PCR Primer Set and Controls Contents
FHV Primer Set
FHV Positive Control (PosC)
Nuclease Free-Water

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25oC). Buffers can be stored for up to 1 year without showing any reduction in performance. The 2x FHV PCR Master Mix, 2x PCR Control Master Mix, FHV Positive Control (PosC) and the Isolation Control (IsoC) should be kept tightly sealed and stored at -20oC for up to 1 year without showing any reduction in performance. Repeated thawing and freezing (> 2 x) should be avoided, as this may reduce the sensitivity. If the reagents are to be used only intermittently, they should be frozen in aliquots