For the rapid isolation and purification of total RNA (including microRNA) and genomic DNA from FFPE tissue samples.

  • Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
  • Convenient spin column protocol 
  • The kit allows for the purification of either pure total RNA, pure genomic DNA or both RNA and genomic DNA together through the use of tailored protease digestion times.
  • Purified RNA and genomic DNA are of the highest quality for use in numerous downstream applications including next generation sequencing, real-time RT-PCR, PCR, mutation screening, and microarray analyses

Items Cat. # Size
FFPE RNA/DNA Purification Kit 25000 50 preps

FFPE RNA/DNA Purification Kit

This kit provides a rapid method for the isolation and purification of total RNA (including microRNA) and DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Alternatively, the kit can be used to isolate total RNA alone or genomic DNA alone from FFPE tissue samples through varying the protease digestion time and performing optional RNase or DNAse digestions.   Nucleic acids recovered with this kit are suitable for further downstrean analysis by PCR, qPCR, microarrays, sequencing etc.  

Norgen Biotek also offers a kit to separate RNA and DNA from the same FFPE tissue sample.  Please visit the product page for more details:  FFPE RNA/DNA Purification Plus Kit #54300  

Background
 
Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. 
 

Kit Specifications
Column Binding Capacity (RNA)
50 μg
Column Binding Capacity (gDNA) 15 μg
Size of RNA Purified All sizes
Maximum Amount of Starting Material:  
Paraffin Slices 5 slices 20 μm thick
Unsectioned Block 25 mg

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year in their unopened containers.

Title The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin.
Journal The Journal of Biological Chemistry. 2014.
Authors Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang
Title Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis.
Journal Cancer Cell. 2013.
Authors Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y.
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Authors Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR.
Title Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Journal Virchows Arch. 2012.
Authors Ludyga N, Grünwald B, Azimzadeh O, Englert S, Höfler H, Tapio S, Aubele M.
Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.
Title Simultaneous recovery of DNA and RNA from formalin-fixed paraffin-embedded tissue and application in epidemiologic studies
Journal Cancer Epidemiology, Biomarkers & Prevention. 2010.
Authors Huang WY, Sheehy TM, Moore LE, Hsing AW, Purdue MP.