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ItemsCat. #Size
Horse (Equine) DNA PCR Detection Kit 61800 24 reactions


Horse (Equine) DNA PCR Detection Kit

In recent years, the adulteration of meat products has become a significant problem in the food industry around the world. In general, the substitution of meat of a different species may not pose harm to the health of consumers. However, meat products consisting of undeclared species such as pig and horse are not desirable in some regions as meats of the aforementioned species are considered taboo either at the cultural or religious level. One of the most publicized recent cases of meat adulteration is the 2013 horse meat adulteration scandal in Europe, where many food products advertised to be beef contained a significant amount of undeclared horse meat. The primary analytical methods utilized to detect meat adulteration are antibody-based detection or nucleic acid-based detection. The use of DNA-based detection has advantages over other methods as DNA can remain stable at high temperatures such that it could be detected from raw or frozen products as well as processed food products.

Norgen’s Horse (Equine) DNA PCR Detection Kit offers a PCR-based detection procedure to detect the presence of horse DNA in food or feed samples. The kit consists of a horse DNA-specific primer mix that is used in conjunction with the provided 2x Real-Time PCR Master Mix to amplify DNA extracted from raw or processed meat samples. A positive control is provided with a known amount of horse DNA (1%) to gauge the level of detection. In addition, a second control primer mix for general meat detection is provided as an internal control and control for PCR inhibition.

Storage Conditions and Product Stability
Upon receipt, store Norgen's Horse (Equine) DNA PCR Detection Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.


Safety Data Sheets: 
ItemsCat. #Size
Horse (Equine) DNA PCR Detection Kit 61800 24 reactions


Horse (Equine) DNA PCR Detection Kit

In recent years, the adulteration of meat products has become a significant problem in the food industry around the world. In general, the substitution of meat of a different species may not pose harm to the health of consumers. However, meat products consisting of undeclared species such as pig and horse are not desirable in some regions as meats of the aforementioned species are considered taboo either at the cultural or religious level. One of the most publicized recent cases of meat adulteration is the 2013 horse meat adulteration scandal in Europe, where many food products advertised to be beef contained a significant amount of undeclared horse meat. The primary analytical methods utilized to detect meat adulteration are antibody-based detection or nucleic acid-based detection. The use of DNA-based detection has advantages over other methods as DNA can remain stable at high temperatures such that it could be detected from raw or frozen products as well as processed food products.

Norgen’s Horse (Equine) DNA PCR Detection Kit offers a PCR-based detection procedure to detect the presence of horse DNA in food or feed samples. The kit consists of a horse DNA-specific primer mix that is used in conjunction with the provided 2x Real-Time PCR Master Mix to amplify DNA extracted from raw or processed meat samples. A positive control is provided with a known amount of horse DNA (1%) to gauge the level of detection. In addition, a second control primer mix for general meat detection is provided as an internal control and control for PCR inhibition.

Storage Conditions and Product Stability
Upon receipt, store Norgen's Horse (Equine) DNA PCR Detection Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Safety Data Sheets: 
 

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