For research use only and NOT intended for In vitro-diagnostics.
A ready-to-use system for the isolation and detection of S. enterica from milk using end-point PCR
|Milk Salmonella enterica PCR Detection Kit||30500||24 Tests|
Milk Salmonella enterica PCR Detection Kit
Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic, Gram-negative bacterium that could survive low temperatures and freezing. It is a rare causative agent of mastitis in cow. However, Salmonella is commonly found in raw milk or dairy products made from unpasteurized milk. In addition, there is an increase in Salmonella contamination in milk after pasteurization, making milk an important vehicle of Salmonella transmission from infected farm animals to humans.
Norgen’s Milk Salmonella enterica PCR Detection Kit is a ready-to-use system for the isolation and detection of S. enterica. First, the kit contains components for the rapid isolation of total DNA, including bacterial DNA, from milk samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains S. enterica Master Mix and controls to allow for PCR Ampification, as well as a Control Master Mix to allow for amplification of both an Isolation Control and a PCR Control. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves (Detection). Please see the flowchart to the right.
The S. enterica Master Mix contains reagents and enzymes for the specific amplification of a 336 bp region of the S. enterica genome. In addition, Norgen’s Milk Salmonella enterica PCR Detection Kit contains a second Master Mix, the Control 2X PCR Master Mix, which can be used to identify possible PCR inhibition and/or inadequate isolation via a separate PCR reaction with the use of the provided PCR Control (PCRC) or Isolation Control (IsoC). This kit is designed to allow for the testing of 24 samples. The Salmonella enterica PCR Primer Set and Controls are also available separately for end-point PCR detection.
Norgen’s Milk Salmonella enterica PCR Detection Kit Contents:
Wash Solution I
Wash Solution II
Elution tubes (1.7 mL)
S. enterica 2x PCR Master Mix
Control 2X Master Mix
Isolation Control (IsoC)
S. enterica Positive Control (PosC)
Norgen’s DNA Marker
Salmonella enterica PCR Primer Set and Controls Contents
S. enterica Primer Set
S. enterica Positive Control (PosC)
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25oC). Buffers can be stored for up to 1 year without showing any reduction in performance. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year when stored at these conditions. The S. enterica 2x PCR Master Mix, Control 2X PCR Master Mix, Isolation Control (IsoC), and S. enterica Positive Control (PosC) should be kept tightly sealed and stored at -20oC. These can be stored for up to 1 year without showing any reduction in performance. Repeated thawing and freezing (> 2 x) of these reagents should be avoided, as this may reduce the sensitivity. If the reagents are to be used only intermittently, they should be frozen in aliquots.
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