For research use only and NOT intended for In vitro-diagnostics.

A ready-to-use system for the isolation and detection of S. enterica from milk using end-point PCR

  • Rapid isolation of high quality DNA from milk
  • Contains two ready-to-use 2X PCR Master Mixes
  • High sensitivity and specificity
  • Includes an isolation control and a PCR control
  • Primer set and controls also available separately
  • Ideal for use in:
    1.  Food Surveillance and Monitoring
    2.  Surveys

Items Cat. # Size
Milk Salmonella enterica PCR Detection Kit 30500 24 Tests

Milk Salmonella enterica PCR Detection Kit

Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic, Gram-negative bacterium that could survive low temperatures and freezing. It is a rare causative agent of mastitis in cow. However, Salmonella is commonly found in raw milk or dairy products made from unpasteurized milk. In addition, there is an increase in Salmonella contamination in milk after pasteurization, making milk an important vehicle of Salmonella transmission from infected farm animals to humans.

Norgen’s Milk Salmonella enterica PCR Detection Kit is a ready-to-use system for the isolation and detection of S. enterica. First, the kit contains components for the rapid isolation of total DNA, including bacterial DNA, from milk samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains S. enterica Master Mix and controls to allow for PCR Ampification, as well as a Control Master Mix to allow for amplification of both an Isolation Control and a PCR Control.  The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves (Detection). Please see the flowchart to the right.

The S. enterica Master Mix contains reagents and enzymes for the specific amplification of a 336 bp region of the S. enterica genome. In addition, Norgen’s Milk Salmonella enterica PCR Detection Kit contains a second Master Mix, the Control 2X PCR Master Mix, which can be used to identify possible PCR inhibition and/or inadequate isolation via a separate PCR reaction with the use of the provided PCR Control (PCRC) or Isolation Control (IsoC). This kit is designed to allow for the testing of 24 samples.  The Salmonella enterica PCR Primer Set and Controls are also available separately for end-point PCR detection.

  • Isolate High Quality, Inhibitor-Free DNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated DNA.
  • Rapid Procedure - Isolate and detect S. enterica in under 3 hours.
  • Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap
  • High Sensitivity and Specificity - The limit of detection is 1000 cfu/mL of milk
  • Purchase Primer Set and Controls Separately - The S. enterica Primer Set, as well as the positive control and negative control can be purchased separately

Linear Range

  • The linear range of Norgen’s Milk Salmonella enterica PCR Detection Kit was determined by analysing a dilution series of a S. enterica quantification standard ranging from 1 x 107 cfu/µl to 1 x 10-1 cfu/µl.
  • Each dilution has been tested in replicates (n = 4) using Norgen’s Milk Salmonella enterica PCR Detection Kit on 1X TAE 1.7% Agarose gel.
  • The linear range of Norgen’s Milk Salmonella enterica PCR Detection Kit has been determined to cover concentrations from 1 x 102 cfu/µl to at least 1 x 106 cfu/µl
  • Under the conditions of the Norgen’s Milk Salmonella enterica DNA Isolation procedure, Norgen’s Milk Salmonella enterica PCR Detection Kit covers a linear range from 1,000 cfu/mL milk to at least 1 x 107 cfu/mL milk

Norgen’s Milk Salmonella enterica PCR Detection Kit Contents: 
Lysis Solution
Binding Solution
Wash Solution I
Wash Solution II
Elution Buffer
Proteinase K
Spin Columns
Collection Tubes
Elution tubes (1.7 mL)
S. enterica 2x PCR Master Mix
Control 2X Master Mix
Isolation Control (IsoC)
S. enterica Positive Control (PosC)
Nuclease Free-Water
Norgen’s DNA Marker
Product Insert
 

Salmonella enterica PCR Primer Set and Controls Contents
S. enterica Primer Set
S. enterica Positive Control (PosC)
Nuclease Free-Water

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25oC). Buffers can be stored for up to 1 year without showing any reduction in performance. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year when stored at these conditions. The S. enterica 2x PCR Master Mix, Control 2X PCR Master Mix, Isolation Control (IsoC), and S. enterica Positive Control (PosC) should be kept tightly sealed and stored at -20oC. These can be stored for up to 1 year without showing any reduction in performance. Repeated thawing and freezing (> 2 x) of these reagents should be avoided, as this may reduce the sensitivity. If the reagents are to be used only intermittently, they should be frozen in aliquots.