Salmonella enterica TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Salmonella enterica TaqMan PCR Detection Kits Price USD$699.00 SKU TM32150 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Salmonella enterica CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic Gram-negative bacterium that could survive low temperatures and freezing. The majority of the 1.3 billion annual cases of Salmonella-caused human gastroenteritis result from ingestion of contaminated food products, such as raw or undercooked meat, seafood, and eggs, as well as raw or unpasteurized milk and dairy products. Salmonella infections are also contracted following consumption of fresh fruits or vegetables that have been contaminated by infected fertilizer. TaqMan PCR Kit Click to expand options Salmonella enterica TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Salmonella enterica TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM32150 (100 preps) Cat. TM32110 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - S. enterica Primer & Probe Mix 280 μL 280 μL S. enterica Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM32150) Salmonella enterica TaqMan PCR Kit - Protocol (100 rxns) (TM32110) Salmonella enterica TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Related Products Salmonella enterica TaqMan PCR Kit Dx Salmonella enterica TaqMan Probe/Primer and Control Set Milk Bacterial DNA Isolation Kit Bacterial Genomic DNA Isolation Kit
Salmonella enterica TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Salmonella enterica TaqMan PCR Detection Kits Price USD$699.00 SKU TM32150 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
